类胰岛素生长因子-1及其受体mRNA在培养肌腱细胞内的表达

THE EXPRESSION OF IGF 1 AND ITS RECEPTOR IN CULTURED TENDON CELL

为了进一步探讨类胰岛素生长因子－１（ＩＧＦ－１）受体系统在培养肌腱细胞群体生命活动中的变化，采用地高辛标记的ＩＧＦ－１及其受体基因的寡核苷酸探针，对原代、第６代及第１３代肌腱细胞ＲＮＡ进行杂交，探测ＩＧＦ－１及其受体在上述细胞中的ｍＲＮＡ表达。结果发现，上述三种细胞均表达ＩＧＦ－１受体ｍＲＮＡ；只有原代和第６代细胞表达ＩＧＦ－１ｍＲＮＡ，而第１３代细胞不表达ＩＧＦ－１ｍＲＮＡ。提示，ＩＧＦ－１ｍＲＮＡ不表达可能是导致第１３代肌腱细胞增殖能力下降的原因之一。

In order to study the expression change of insulin like growth fator 1 (IGF 1) and its receptor genes in different generations of tendon cell in culture, Dig labeled synthesized oligonucleotide probes were used to detect the mRNA expression in primary, 6th and 13th generation of tendon cell. The results showed that IGF 1 recpetor mRNA was expressed in all of the 3 above generation tendon cells. IGF 1 mRNA was expressed only in primary and 6th generationn cells. Tendon cell of 13th generation did not express IGF 1 mRNA. It might suggest that the absence of IGF 1 mRNA expression be one of the causes which led to the decrease of reproductive ability of 13th generation tendon cell.