摘要
为了进一步探讨类胰岛素生长因子-1(IGF-1)受体系统在培养肌腱细胞群体生命活动中的变化,采用地高辛标记的IGF-1及其受体基因的寡核苷酸探针,对原代、第6代及第13代肌腱细胞RNA进行杂交,探测IGF-1及其受体在上述细胞中的mRNA表达。结果发现,上述三种细胞均表达IGF-1受体mRNA;只有原代和第6代细胞表达IGF-1mRNA,而第13代细胞不表达IGF-1mRNA。提示,IGF-1mRNA不表达可能是导致第13代肌腱细胞增殖能力下降的原因之一。
In order to study the expression change of insulin like growth fator 1 (IGF 1) and its receptor genes in different generations of tendon cell in culture, Dig labeled synthesized oligonucleotide probes were used to detect the mRNA expression in primary, 6th and 13th generation of tendon cell. The results showed that IGF 1 recpetor mRNA was expressed in all of the 3 above generation tendon cells. IGF 1 mRNA was expressed only in primary and 6th generationn cells. Tendon cell of 13th generation did not express IGF 1 mRNA. It might suggest that the absence of IGF 1 mRNA expression be one of the causes which led to the decrease of reproductive ability of 13th generation tendon cell.
出处
《中国修复重建外科杂志》
CAS
CSCD
1997年第6期369-371,共3页
Chinese Journal of Reparative and Reconstructive Surgery
基金
国家自然科学基金
关键词
IGF-1
肌腱细胞
受体
MRNA
培养
Tendon cell Insulin like growth factor 1 Receptor Expression