纯钛种植体—骨界面的飞行时间二次离子质谱分析

ANALYSIS OF PURE TITANIUM IMPLANTS BONE INTERFACE BY TOF SIMS

为探明纯钛与骨组织间的相互作用与结合机理，采用荧光显微镜及先进的飞行时间二次离子质谱分析仪，对已形成骨整合的１，３及６个月钛—骨结合界面进行了分析和测试。结果表明，纯钛种植体与骨结合十分紧密；钛与骨组织间的离子作用是相互的；钛—骨间既存在物理性结合，也存在化学性结合。钛离子向组织内有扩散，但扩散的量极微，扩散的深度可达１００μｍ以上；钛离子的扩散在植入骨组织后早期（１个月～３个月）较多，以后较少。钛离子向组织内沥出的同时，组织内Ｃａ＋、ＯＨ－、Ｏ－等离子向钛氧化膜内也有渗透。对纯钛种植体周围骨组织的生长方式观察，证明呈双向性生长，一种是骨组织从邻近或远离种植体的骨床向种植体表面生长；另一种是新骨组织直接沉积在种植体表面并向骨床生长。研究根据纯钛种植体与骨组织间的离子分布、渗透等情况，从分子、原子水平进一步对钛与骨组织的相互作用及结合机理进行了探讨。

In order to study the Titanium bone interaction and integration mechanism, the titanium implant was implanted in the tibia of 9 Newzealand rabbits, and the Ti bone interface performed for 1, 3, and 6 months were examined and analyzed by fluorescence microscope and advanced TOF SIMS techniques. The results showed that Ti bone tissue was integrated closely in a very reactive manner. Both physical and chemical integration occured in the Ti bone interface. The Ti bone could diffuse into the bone tissue though the diffusion was very limited. It was up to 100 μm in depth during the early period. The diffusion density was high, and later in a smooth distribution. Furthermore, while Ti + diffused into the bone tissue, other elements such as Ca +, OH -, O - , etc, could also diffuse into titanium in exchange. The growth pattern around the bone tissue was in two fashions, one was implantefugal and the other was implantopetal. In this study, based on the ionic distribution, osmosis and impurity elements distribution, the Ti bone integration mechamism was discussed at molecular and atomic level.