生长因子对人精原干细胞体外存活和增殖的影响

Growth Factors Promote the Survival and Proliferation of Human Spermatogonium Stem Cells in vitro

目的:探讨3种生长因子——干细胞因子(SCF)、白血病抑制因子(LIF)和碱性成纤维细胞生长因子(bF-GF)对精原干细胞(SSCs)体外存活及增殖的作用。方法:在培养液中加入不同浓度的SCF、LIF和bFGF,各生长因子均设浓度为0、5、10、20μg/L4个浓度组,每种生长因子不同浓度1式3份,每隔8～12h观察细胞的体外存活及增殖情况。应用光镜、电镜观察SSCs的形态结构特点。结果:培养液中加入不同浓度的SCF、LIF及bFGF后,SSCs的存活时间均比对照组有所延长,组间比较差异有显著性(P<0.05)。结论:生长因子SCF、LIF和bF-GF等可促进SSCs体外存活及增殖。

Objective： To study the effects of growth factors on the survival and proliferation of human spermatogonial stem cells （SSCs） in vitro. Methods ： SSCs were treated with the growth factors SCF, LIF and bFGF added to the culture, each at the concen- trations of 0, 5, 10 and 20 ttg/L and repeated three times. The survival time and proliferation rate of the cells were determined every 8-12 hours and their morphological features observed with the light microscope and electron microscope. Results： The survival time and proliferation rate of the SSCs were significantly increased in the treated groups as compared with the control （ P 〈 0. 05 ）. Conclusion ： The growth factors SCF, LIF and bFGF can promote the survival and proliferation of SSCs in vitro. Natl J Androl, 2008, 14 （10） ： 876-878