前列腺癌患者10号染色体杂合性缺失与骨转移关系的初步研究

Loss of Heterozygosity on Chromosome 10 Is Associated with Bone Metastasis of Human Prostate Cancer

目的:探讨人前列腺癌特异性骨转移的细胞遗传学机制。方法:应用比较基因组杂交技术对18例前列腺癌患者进行染色体变异情况的初步分析,确定可能与骨转移密切相关的变异染色体。再应用PCR及微卫星多态性技术重点对10号染色体上的7个微卫星位点进行杂合性缺失(LOH)检测。结果:11例伴有远处骨转移的患者组织样本中,10号染色体的变异率为90.9%(10/11),显著高于其他染色体(P<0.01);进一步分析发现,7个微卫星位点的LOH现象中,骨转移患者的发生率最高,且以D10S1693～D10S587(10q24.2～q25.3)区的LOH发生率最高。结论:10号染色体上D10S1693～D10S587区(10q24.2～q25.3)是前列腺癌骨转移患者中一个高频的LOH区,此区可能与前列腺癌患者远处骨转移的发生密切相关。

Objective ： To study the cytogenetic mechanism of bone metastasis of human prostate cancer （PCa）. Methods： We analyzed chromosome variation by comparative genomic hybridization in 18 patients with prostate cancer to determine the chromosome vari- ants associated with bone metastasis, and focused on 7 mierosatellite sites on chromosome 10 for the detection of the loss of heterozygosity （LOH） by PCR-based microsatellite polymorphism analysis. Results： In the 11 samples with bone metastasis, the variation rate of chromosome 10 was 90.9% （ 10/11 ）, significantly higher than that of the others （ P 〈 0.01 ）. A much higher LOH frequency was observed at the 7 microsatellite loci on chromosome 10 and the highest located in 10q24.2-q25.3 （ D10SI693-D10S587 ） in the PCa patients with bone metastasis. Conclusion ： There is a high-frequency LOH region in 10q24.2-q25.3 （ D10S1693-D10S587 ） on chromosome 10 in PCa patients with bone metastasis, which may be potentially involved in PCa progression and specific bone metastasis. Natl J Androl, 2008, 14 （10） ： 879-883