摘要
目的:探讨人前列腺癌特异性骨转移的细胞遗传学机制。方法:应用比较基因组杂交技术对18例前列腺癌患者进行染色体变异情况的初步分析,确定可能与骨转移密切相关的变异染色体。再应用PCR及微卫星多态性技术重点对10号染色体上的7个微卫星位点进行杂合性缺失(LOH)检测。结果:11例伴有远处骨转移的患者组织样本中,10号染色体的变异率为90.9%(10/11),显著高于其他染色体(P<0.01);进一步分析发现,7个微卫星位点的LOH现象中,骨转移患者的发生率最高,且以D10S1693~D10S587(10q24.2~q25.3)区的LOH发生率最高。结论:10号染色体上D10S1693~D10S587区(10q24.2~q25.3)是前列腺癌骨转移患者中一个高频的LOH区,此区可能与前列腺癌患者远处骨转移的发生密切相关。
Objective : To study the cytogenetic mechanism of bone metastasis of human prostate cancer (PCa). Methods: We analyzed chromosome variation by comparative genomic hybridization in 18 patients with prostate cancer to determine the chromosome vari- ants associated with bone metastasis, and focused on 7 mierosatellite sites on chromosome 10 for the detection of the loss of heterozygosity (LOH) by PCR-based microsatellite polymorphism analysis. Results: In the 11 samples with bone metastasis, the variation rate of chromosome 10 was 90.9% ( 10/11 ), significantly higher than that of the others ( P 〈 0.01 ). A much higher LOH frequency was observed at the 7 microsatellite loci on chromosome 10 and the highest located in 10q24.2-q25.3 ( D10SI693-D10S587 ) in the PCa patients with bone metastasis. Conclusion : There is a high-frequency LOH region in 10q24.2-q25.3 ( D10S1693-D10S587 ) on chromosome 10 in PCa patients with bone metastasis, which may be potentially involved in PCa progression and specific bone metastasis. Natl J Androl, 2008, 14 (10) : 879-883
出处
《中华男科学杂志》
CAS
CSCD
2008年第10期879-883,共5页
National Journal of Andrology
基金
北京市科干局科研基金
关键词
前列腺癌
骨转移
染色体
杂合性缺失
prostate cancer
bone metastasis
chromosome
loss of heterozygosity