两种精子分析系统的比较研究

Comparison of the Parameters Obtained by Sperm Quality Analyzer V and Computer-aided Sperm Analysis System

目的:评估自动化精子质量分析仪SQA-V与计算机辅助精液分析(CASA)系统之间各主要参数的差异性及其在不育和生育男性精液质量分析中的应用。方法:用SQA-V和CASA系统分别检测12例正常生育者和73例不育患者新鲜精液标本,分别分析精子密度、精子活动率、活动精子浓度、精子活动力指数、精子头侧向位移、鞭打频率、精子曲线运动速度、精子前向运动速度、平均路径速度、直线性及前向性运动速度等参数,针对两者进行相关性分析。结果:不育组和生育组男性各项指标间存在显著差异,两种分析系统检测的精子密度之间(r=0.58,P<0.01)、活动精子浓度之间(r=0.75,P<0.01)、平均精子速度(SQA-V)和精子路径速度(CASA)之间(r=0.59,P<0.01)具有较好的一致性,SQA-V的活动精子指数(SMI)和CASA的前向性、曲线运动速度、精子运动的直线性、前向运动速度、平均路径速度、鞭打频率等参数具有显著相关性(P<0.05)。结论:SQA-V和CASA系统比较,在各主要参数上具有较好的一致性,能够反映临床不同组群患者精液的差异性。

Objective： To assess the differences between the main parameters obtained by sperm quality analyzer V （SQA-V） and computer-aided sperm analysis system （ CASA）, and to investigate their application to sperm quality analysis for fertile and infertile men. Methods： Twelve fresh semen samples from fertile volunteers and 73 from infertility patients were detected with SQA-V and CASA for sperm concentration and motility, the percentage and concentration of motile sperm, sperm motility index （SMI）, amplitude of lateral head displacement, （ ALH）, beat cross frequency （ BCF）, curvilinear velocity （VCL） , straight line velocity （ VSL）, average path velocity （VAP）, linearity （LIN = VSL/VCL） and straightness （STR = VSL/VAP）. The correlation between the parameters obtained by the two devices were analyzed. Results： Significant differences were observed in the above parameters between the fertile and infertile groups. An obvious consistency was noted between the results from SQA-V and those from CASA in sperm concentration （ r = 0.58, P 〈 0.01）, motile sperm concentration （r = 0.75, P 〈 0.01） and average sperm velocity （r = 0.59, P 〈 0.01）. Significant correlations were found between the SMI from SQA-V and STR, LIN, BCF, VCL, VSL and VAP from CASA （P 〈 0.05）. Conclusion： There is a consistency between the results from SQA-V and those from CASA. Both the devices can detect the seminal differences between different cohorts of patients. Natl J Androl, 2008, 14 （10） ： 923-926