感染广州管圆线虫小鼠脑组织的病理变化观察

Pathological Change in the Brain of Mice Infected with Angiostrongylus cantonensis

目的观察小鼠感染广州管圆线虫后脑组织的病理学改变。方法以广州管圆线虫Ⅲ期幼虫人工感染BALB/c小鼠48只,每鼠经口感染40条幼虫,在感染后第7、10、13、16、19、22、25和28天分别解剖6只小鼠。取脑组织,切片后HE染色,观察脑组织病理改变,计数脑组织内虫体。各组均设平行对照健康小鼠1只。结果小鼠感染广州管圆线虫Ⅲ期幼虫后,第10天肉眼下可从小鼠脑组织中检获虫体,但数量较少,平均为(7±1.73)条;感染后第16天,检获虫体最多,平均为(23.66±4.93)条;随后随感染时间的延长而逐渐减少。小鼠感染第15天时出现明显的神经系统症状,多数小鼠在感染后第22天死亡。病理切片观察可见脑组织有机械性损伤,脑实质中可见空洞及炎症反应。脑膜及蛛网膜下腔可见虫体,第13天即可见脑膜炎症反应,并随感染时间延长而加重。结论小鼠感染广州管圆线虫Ⅲ期幼虫可引起脑组织病理改变,并随感染时间延长逐步加重。

Objective To observe the pathological change in the brain of Angiostrongylus cantonensis-infected mouse. Methods Forty-eight mice were orally infected each with 40 third stage larvae of A. cantonensis, 3 mice were sacrificed at 7, 10, 13, 16, 19, 22, 25, 28 days postinfection respectively for worm recovery, and another 3 mice were for observing the histopathological change in tissue sections of the brain. Results Ten days postinfection, worms were found in the brain of the infected mice with a mean worm number of （7.0±1.7） per mouse. The highest number of worms was found at 16 days postinfection, with a mean of （23.7±4.9） per mouse. Notable symptoms of nervous system were seen on 15 days postinfection. Most mice died around 22 days postinfection. Histological examination revealed mechanical damages. Cavitis and inflammation were observed in the brain parenchyma. Worms were seen in the subarachoid space. Meningitis-like signs started at 13 days and aggravated then. Conclusions Infection of A. cantonensis causes pathological change in mouse brain and the process is aggravating with postinfection time.