心动过速性心肌病动物模型的建立

To establish an experimental model of tachycardiomyopathy.

目的在非X线引导下,对猪快速右心房起搏构建心动过速性心肌病动物实验模型。方法将15头滇南小耳猪,按性别相同、体重和月龄相近等条件,划分为3个组,每组5头猪,每组采用下述三种处理方案之一:(1)起搏1周组:以AOO方式,起搏频率为240次/分,起搏右心房;(2)起搏4周组:按上述方法起搏;(3)假手术组:植入起搏电极和起搏器,但不打开起搏器行快速起搏。观察三组猪心动过速性心肌病发病过程中不同时期的表现,利用光学显微镜观察心肌细胞结构及间质结构变化。结果起搏4周后,实验猪均出现明显呼吸困难、进食减少、四肢浮肿等心力衰竭表现。光学显微镜下可见心肌实质和间质发生明显重构。结论快速右心房起搏是构建心动过速性心肌病模型的有效方法。

Objective To establish an experimental model of tachycardiomyopathy by rapid right atrial pacing at 240 beats per minute in pigs. Methods Fifteen Diannan pigs were equally divided into 3 groups. Each groups were received one of the three managements： rapid atrial pacing （240 beats/min） for 1 week, rapid atrial pacing for 4 weeks, and sham-operated control. Then, we observed the changes of myocardial remodeling by light microscopic examination and naked eyes. Results As pacing prolonged, the pigs developed congestive heart failure as evidenced by dyspnea, ascites and peripheral edema within 4 weeks of activating the pacemaker. Postmortal examination and light microscopy revealed tachycardiomyopathy characterized with biventricular cardiac dilation, biventricular wall thickness and the myocardial remodeling. Conclusion Tachycardiomyopathy can successfully be established by rapid right atrial pacing in the pigs.