炎性因子对妊娠相关血浆蛋白A分泌的影响

The influence of inflammation factors on secretion of pregnancy-associated plasma protein-A

目的研究炎性因子白细胞介素-6（IL-6）和白细胞介素-1β（IL-1β）对人冠状动脉平滑肌细胞（HCASMC）分泌妊娠相关血浆蛋白A（PAPP—A）的影响。方法一期：分别加入20μg／L的IL-1β（IL-1β组）、10μg／L的IL-6（IL-6组）和0μg／L的炎性因子（对照组），孵育2、4、8、24、36h后，分别收集细胞上清液；二期：分别采用IL-1β（0、5、20、40μg／L）和IL-6（0、5、10、50μg／L）刺激HCASMC，6h后收集细胞和细胞上清液。采用ELISA法检测细胞上清液内PAPP—A的表达量。结果一期：IL-1β组PAPP—A表达量在2h时开始增加，8、24、36h其浓度显著高于对照组，并随时间的延长而不断增加；IL-6组PAPP—A的表达量在2h开始增加，4、8、24、36h其浓度显著高于对照组，并随时间的延长而不断增加。二期：随着IL-1β和IL-6剂量的增加，PAPP—A的表达量不断升高，其中TL-1β组20μg／L和40μg／L均显著高于0μg／L时的浓度；IL-6组10μg／L和50μg／L均显著高于0μg／L时的浓度。结论IL-1β和IL-6可使HCASMC分泌PAPP—A增加，并呈时间和剂量依赖性。

Objective To observe the effect of IL-1β and IL-6 on secretion of pregnancy-associated plasma protein A（PAPP-A） in humam coronary atery smooth muscle cell（HCASMC）. Methods At the first stage, HCASMCs were incubated with IL-1β （20 μg/L）, IL-6 （10 μg/L） and inflammation factor（0 μg/L,served as the control group）. Culture media were collected after 2, 4, 8, 24 and 36 h. At the second stage： HCASMCs were stimulated by IL-1β（0, 5, 20, 40 μg/L） and IL-6（0,5,10,50 μg/L）;0 μg/L served as the control group. Culture media were collected after 6 h. The concentrations of PAPP-A in culture media were measured by ELISA. Results At the first stage,incubation of HCASMC with 20 μg/L of IL-1β and 10 μg/L of IL-6 resulted in increase in secretion of PAPP-A which increased continuously with time. In the group cultured with IL-1β,it began to increase at 2 h, and the concentrations at 8,24 and 36 h were higher than those in the control group significantly. In the group cultured with IL-6, it began to increase at 2 h,and the concentrations at 4, 8,24,36 h were higher than those in the control group significantly. At the second stage,incubation of HCASMC with different concentrations of IL-1β and IL-6 caused dosedependent increase in PAPP-A secretion. In the group cultured with IL-1β,the PAPP secretion in both 90 μg/L and 40 μg/L groups exceeded the control group significantly. In the group cultured with IL-6,the PAPPsecretion in both 10 μg/L and 50 μg/L groups exceeded the control group significantly. Conclusions IL-1β and IL-6 can induce the secretion of PAPP-A by HCASMCs,implicating that inflammation may play an important role in the development of ACS.