八肽胆囊收缩素与血管紧张素Ⅱ在拮抗大鼠吗啡镇痛中的协同作用

SYNERGISTIC EFFECT OF CHOLECYSTOKINlN OCTOPEPTIDE AND ANGIOTENSIN Ⅱ IN ANTAGONIZING MORPHINE-INDUCED ANALGESIA IN RATS

以往的研究表明，八肽胆囊收缩素（CCK－8）和血管紧张素Ⅱ（AⅡ）均为有效的抗阿片物质。大鼠脑室（i．C．V．）注射CCK－8或AⅡ均能拮抗吗啡的镇痛作用。本研究的目的是观察CCK－8与AⅡ在桔抗吗啡镇痛时是否相互协同。在给大鼠皮下注射吗啡10min后，再i．c．v．单独注射CCK－8或AⅡ，或同时注射二者不同剂量和不同比例的混合物。结果表明，联合应用CCK－8与AⅡ所产生的桔抗吗啡镇痛的作用明显大于单独使用CCK－8或AⅡ。应用两因素重复设计的方差分析对结果进行统计学处理，证实CCK－8和AⅡ在一定的比例范围内联合应用，确实能够协同拮抗吗啡的镇痛作用。

Our previous studies have shown that both cholecystokinin octopeptide(CCK-8)and angiotensin Ⅱ (A Ⅱ) are potent anti-opioid substances. Intracerebroventricular(i.c. v. ) injection of CCK-8 or A Ⅱ dose-dependently antagonizes morphine-induced analgesia(MIA). In the present study, we observed the combined effect of CCK-8 and A Ⅱ in antagonizing MIA. CCK-8 and AⅡ were injected i. c. v. to rats in various proportions and doses. The results were analyzed with two-way analysis of variance. An optimal proportion and dosage were identified. The combined injection of CCK-8 and A Ⅱ in a ratio of 1 ng: 2. 5μg or 1 ng: 5μg ratio produced a synergistic effect in antagonizing MIA.