染锰大鼠脑匀浆生化分析

Biochemical analysis of brain in manganese-exposure rats

目的了解锰对脑组织相关酶类活性的影响及可能的细胞保护机制。方法大鼠经饮水连续染锰(MnCl2.4H2O0.5g/L和5.0g/L)60d后,应用荧光光度比色法测定新鲜脑组织匀浆的谷丙转氨酶(ALT)、谷草转氨酶(AST)、碱性磷酸酶(ALP)、胆碱酯酶(ChE)、单胺氧化酶(MAO)、氧化亚氮合酶(NOS)、超氧化物歧化酶(SOD)活性和谷胱甘肽(GSH)、丙二醛(MDA)、蛋白质(PRO)含量,并设生理盐水对照组。结果与对照组比较,高、低剂量组的ALT、AST、ALP、ChE、MAO、NOS活性和MDA含量明显升高;而SOD活性和GSH含量则显著降低。结论过量锰沉积可影响脑组织多种酶类的正常代谢,对脑组织造成毒性损害。

Objective To study the effects of manganese on enzymes and possible cellular defense mechanism. Methods Male SD rats were given drinking water containing manganese chloride （ MnCl2·4H2O 0.5 g/L and 5.0 g/L respectively） for 60 days. At the end of experiment, all the rats of the manganese-exposed groups and control group were decapitated after anaesthesia with 2% sodium phenobarbitone intraperitoneally, the brains were removed and activities of alanine aminotransferase（ALT）, aspartate aminotransferase （ AST）, alkaline phosphate （ ALP）, cholinestarase （ ChE ）, monoamine oxidase （ MAO ）, superoxide dismutase （ SOD ）, nitric oxide synthase（NOS）, and levels of glutathione （GSH）, maleic dialdehyde （MDA）, and proteins were biochemically analyzed. Results Compared with the control group, activities of ALT, ALT, ALP, ChE, MAO, NOS and MDA level increased, but the SOD activity and GSH level decreased significantly in 2 manganese-exposed groups. Conclusion Manganese induced toxic effects on brains in rats by interfering with the metabolism of related enzymes.