HPLC法测定微泵给药后头孢他啶血药浓度

Determination the Concentration of Ceftazidime in Plasma After Using Minipump by HPLC

目的:建立高效液相色谱法测定头孢他啶的人体血药浓度。方法:色谱柱为HypersiI C_(18)(250 mm×4.6mm,10μm)柱;流动相为甲醇-0.02 mol·L^(-1)磷酸二氢钾(13.5:86.5),流速为1 ml·min^(-1);检测波长为254 nm;柱温30℃;采用饱和硫酸铵溶液直接沉淀蛋白后进样。结果:头孢他啶血药浓度与峰面积线性关系良好;线性范围1.0～60μg·ml^(-1)(r=0.9997)。日内、日间RSD<5%:头孢他啶高、中、低浓度的平均方法回收率分别为99.2%,99.1%,98.6%,平均提取回收率分别为42.3%,42.0%,43.6%。结论:该法操作简便、准确、快速,回收率稳定,适用于头孢他啶体内动力学研究。

Objective： To establish an HPLC method for the determination of Ceftazidime in plasma. Method： The Hypersil C18 column （250 mm ×4.6 mm, 10μm） was used. The mobile phase was methanol-potassium dihydrogen phosphate （13.5：86.5） and flow rate was 1 ml.min^-1. The detection wavelength of UV was at 254 nm and column temperature was 30℃. The sample was deposited by saturated ammonium sulfate solution and then injected. Result： Standard curve was linear in the concentration range of 1.0 - 60 μg . ml^- 1 （ r = 0.999 7 ）. The relative standard deviations of within-day and between-day less than 5.0%. the average method recovery rate of three concentration were 99.17% ,99.07% and 98.62% respectively, the average extraction recovery rate of three concentration were 42.29% ,42.02% and 43.62% respectively. Conclusion： The operation apppeared to be simple, accurate and precise. The method had stable rate of extraction recovery. It was suitable for the pharmaeokinetics study of ceftazidime.