反相高效液相色谱法测定盐酸异丙肾上腺素注射液的含量

Determination of lsoprenaline Hydrochloride Injection by RP-HPLC

目的:建立HPLC法测定盐酸异丙肾上腺素注射液的含量。方法:采用Diamonsil^(TM) C_(18)色谱柱(250 mm×4.6 mm,5μm),流动相为0.005 mol·L^(-)庚烷磺酸钠(pH 3.0)-甲醇(65:35),流速1.0 ml·min^(-1),检测波长280 nm,柱温35℃。结果:盐酸异丙肾上腺素在0.02～2 mg·ml^(-1)范围内线性关系良好(r=0.999 9,n=7),平均回收率为99.7%(n=9)。结论:本方法简便、快速、准确,专属性好,可用于盐酸异丙肾上腺素注射液的含量测定。

Objective： To develop an RP-HPLC method for determination the content of Isoprenaline Hydroehloride Injection. Method： DiamonsilTM C18 column （250 mm×4.6 mm, 5μm） was used, the mobile phase was 0.1 mol.L^-1 sodium heptane sulfonate （pH 3.0）-methanol （45： 55）, flow rate was 1.0 ml.min^-1. The UV detection wavelength was 280 nm, and the column temperature was 35 ℃. Result： The linear range was 0.0：2 · 2 mg· ml^-1 （ r = 0. 999 9, n = 7 ） and the average recovery was 99.7% （ n = 9 ） for isoprenaline hydroehloride. Conclusion ： The method proposed for determination the content of Isoprenaline Hydroehloride Injection is simple, rapid, accurate and specific.