摘要
本实验研究紫甘薯花色苷色素对金黄色葡萄球菌的抑制作用。结果表明,紫甘薯花色苷色素对金黄色葡萄球菌抑制的最低浓度为200μg/ml。金黄色葡萄球菌生长曲线和透射电镜观察表明,紫甘薯花色苷的抑菌作用可能是通过增强细胞膜的通透性,使细胞异常生长,抑制对数生长期的细胞分裂,使细胞质稀薄、解体,导致细胞死亡。SDS-PAGE分析表明,紫甘薯花色苷对大肠杆菌和金黄色葡萄球菌蛋白表达影响不明显,未见特征性条带的消失,仅对部分蛋白质合成量有影响。
The reults of bacteriostatic experiment of anthocyanins from purple sweet potato indicated that their minimum inhibitory concentration （MIC） on Staph.aureus is 200ug/ml. The observation under transmission electron microscope （TEM） was carried out and the growth curve of Staph.aureus was analyzed. It was indicated that the anthocyanins might change the permeability of cell membrane and make cell excrescent to inhibit cell division. As a result, cytoplasm becomes thin, disassembles and even dies. SDS-PAGE analysis suggested that the anthocyanins have some effects on synthesized quantifies of some proteins, but no disappearence of special protein lines is found.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2008年第10期59-62,共4页
Food Science
基金
江苏省高技术项目(BG2004323)
