酶解牛蒡提取膳食纤维工艺研究

Study on Extraction Technology of Dietary Fiber from Burdock by Enzymolysis Method

本实验采用酶法提取牛蒡膳食纤维,利用植物蛋白酶和糖化酶去除牛蒡蛋白质和淀粉,用95%乙醇沉淀可溶性膳食纤维,过滤后用乙醇、丙酮洗涤滤渣,以去除脂肪等脂溶性物质,干燥,灰化,残渣扣除灰分即得膳食纤维。结果表明,酶法提取牛蒡膳食纤维的最佳工艺条件:植物蛋白酶和糖化酶加酶量分别为6%和1.2%,pH7.0,时间4h,温度50℃,在此条件下膳食纤维提取率为63.74%。

Dietary rider was extracted from burdock hydrolyzed by enzymes in this study. The plant protease and glucoamylase were used to hydrolyze respectively protein and starch after pretreating burdock. The soluble dietary fiber was precipitated by using 95% ethanol and then washed by using ethanol and acetone to remove fat-soluble substances. After drying and ashing, the residue was purifed dietary fiber. The results showed that the optimal techological conditions for preparing burdock dietary fiber are as follows： plant protease amount 6%, glucoamylase 1.2%, pH 7.0, time 4 h and temperature 50℃. The yield of the burdock dietary fiber is 63.74%.