响应面分析优化壳聚糖酶产生菌的发酵条件

Optimization of Fermentation Conditions of Chitosanase-producing Aspergillus fumigatus K1 by Using Response Surface Analysis

采用响应面分析对影响烟曲霉K1(Aspergillus fumigatus K1)生产壳聚糖酶的发酵培养基进行优化。首先采用部分因素试验筛选出影响壳聚糖酶产量的显著因素:牛肉膏和CaCl2。利用最陡爬坡路径逼近最大产酶区域。最后,应用中心组合设计和响应面分析确定烟曲霉K1产壳聚糖酶的最佳培养基组成(%,W/V)为:壳聚糖1.5、牛肉膏2.1、KH2PO40.1、MgSO4·7H2O0.1、CaCl20.4和Tween0.1。经过优化,壳聚糖酶产量可达44.24U/ml,比开始条件提高2.4倍以上。

Response surface analysis was used to optimize the fermentation medium for chitosanase production by Aspergillusfumigatus K 1. In the first step, fractional factorial design was adopted to screen out the important factors affecting chitosanase production, which were beef extract and CaCh. The path of the steepest ascent was utilized to approach the optimal region of the chitosanase production. At last, the optimal composition of the fermentation medium was obtained by central composite design and response surface analysis for chitosanase production by Aspergillus fumigatus K1. The optimal culture medium （%, W/V） consisits of ： chitosan 1.5, beef extract 2.1, KH2PO4 0.1, MgSO4 · 7H2O 0.1, CaCl2 0.4 and Tween 0.1. After optimization, the yield of chitosanase production is 44.24 U/ml, 2.4 times higher than that in the original medium.