反相高效液相色谱测定不同品种皱皮木瓜中齐墩果酸和熊果酸含量

Determination of Oleanolic Acid and Ursolic Acid in Different Species of Fructus chaenomeles by RP-HPLC

采用反相高效液相色谱法测定鲜皱皮木瓜中齐墩果酸和熊果酸含量,并进行方法学考察。采用AgilentC18柱(4.6mm×250mm,5μm);流动相:甲醇-1%HAc水溶液(85:15);流速:0.6ml/min;柱温:35℃;检测波长:210nm。齐墩果酸在0.162～6.48μg范围内线性良好,回归方程为y=512802x+19211(R2=0.9997),平均回收率分别为100.2%,RSD为0.9%(n=6);熊果酸在0.391～15.62μg范围内线性良好,回归方程为y=50865x-5090.4(R2=0.9998),平均回收率为100.0%,RSD为1.1%。该方法简便准确,重现性好,适用于鲜皱皮木瓜中齐墩果酸和熊果酸的定量分析。

A method for determination of oleanolic acid and ursolic acid in fresh Fructus chaenomeles was established. The determination by RP-HPLC was carried out on Agilent C18（4.6 mm× 250 mm, 5 u m） column. The mobile phase consisted of methanol-0.5% HAc water （85：15） with the flow rate of 0.6 ml/min. The column remained at 35 ℃ and the detection wavelength was at 210 nm.The calibration curve is linear in the range of 0.162-6.48ug for oleanolic acid （R^2= 0.9997）, with the regression equation being y= 512802x＋19211. The average recovery is 100.2% （RSD=0.9%, n=6）. The calibration curve was linear in the range of 0.391 - 15.62 ug for ursolic acid （R^2= 0.9998）, with the regression equation being y = 50865x - 5090.4. The average recovery was 100.0% （RSD=1.1%, n=6）. These conclusions showed that this method is simple, accurate and reproducible for quantitative analysis of oleanolic acid and ursolic acid in fresh Fructus chaenomeles.