摘要
对"恩五叶蜜"干叶采用苷元提取法提取得粗苷元后,再于丙酮中重结晶得苷元,层析分离得单体苷元,质谱仪检测,得到3,7,12-羟基-24-乙酰基皂苷元分子量435D,最大可能分子式C26H43O5,在17号位结合的辛烷烃上有酯基团;7,8-羰基-11-羟基-3-乙酰基苷元分子量501D,最大可能分子式为C32H53O4,在3号位上结合酯基团,二者苷元与绞股蓝原变种苷元母核相同,是典型人参皂苷元,前二者在17号位或15号位上结合辛烷烃,后者在17号位上都结合辛烯烃,可能揭示其不苦的原因。
The crude sapogenins extracted from dried "Enwuyemi" Gynostemma pentaphyllum leaves were recrystallized and separated by Sephadex G-15 to obtain sapogenin monomers. According to the detection results by mass spectrometer, the obtained 3,7,12-hydroxy-24-acetyl-sapogenin is of the molecular weight 435 D and the most possible molecular formula C26H4305, whose 17th place is combined with octane having ester group. And 7-8-carbonyl-11-hydroxy-3-acetyl-sapogenin is also found, whose molecular weight and most possible molecular formula are 501 D and C32H5304 respectively, and whose 34 place is combined with ester group. Both of them are identical with the parent nucleus of original variant of Gynostemrna pentaphyllum sapogenins, and are tipical ginseng sapogenins. The combination of 17th or 15^th place ofthe two formers with octane and the combination of 17thplace of the latter with octane might reveal the reason of their no bitterness.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2008年第10期525-527,共3页
Food Science
基金
生物资源保护与利用湖北省重点实验室资助项目(2007021)
关键词
甜味绞股蓝
皂苷元
分离鉴定
"Enwuyemi" Gynostenma pentaphyllum
sapogenins
isolation and identification