非抑制型电导检测离子排斥色谱法测定江西白酒中的有机酸

Determination of Organic Acids in Jiangxi Wines by Nonsuppressed Ion Exclusion Chromatography with Conductometric Detection

建立了非抑制型电导检测离子排斥色谱法分离并测定6种有机酸(草酸、酒石酸、苹果酸、甲酸、琥珀酸、乙酸)的方法。以Ion Pac ICE-AS6色谱柱为分离柱,选用背景较低的稀苯甲酸溶液为淋洗液,考察了淋洗液浓度、流速、色谱柱温度对分离和测定有机酸的影响。通过实验确定最佳的色谱条件为:1.0mmol/L苯甲酸溶液作为淋洗液,流速0.45ml/min,柱温35℃。所测有机酸的检测限为0.0341～0.9045mg/L,相对标准偏差在0.13%～5.12%之间(n=5),回收率为90.75%～108.5%,方法具有较好的准确度和精密度。该法用于江西5个不同生产厂家23个白酒样品中有机酸的测定,结果令人满意。

A simple and sensitive method was established for the separation and detection of 6 organic acids （oxalic acids, tartaric acid, malic acid, formic acid, succinic acid and acetic acid） by nonsuppressed ion exclusion chromatography with conductometric detection. The effect of benzoic acid concentration, flow rate and column temperature on the separation of organic acids was investigated by using Ion Pac ICE-AS6 as analytical column and diluted benzoic acid of lower background as eluent. Through the test, the optimal separation of six organic acids was achieved with 1.0mmol/L benzoic acid solution as the eluent at a flow rate of 0.45 ml/min, and column temperature at 35 ℃. Detection limits for the organic acids ranged from 0.0341 to 0.9045 mg/L, the relative standard deviations were between 0.13% and 5.12%, and the recovery rates were between 90.75% and 108.5%, the measuring method was provided with better accuracy and precision. The organic acids in 23 Jiangxi wine samples from the five different manufacturers were analyzed with satisfactory results.