油菜蜂花粉对大鼠酒精性肝损伤防治的研究

Study on Preventive and Curative Effects of Rape Bee Pollen Extracts on Alcoholic Liver Disease of Rats

观察油菜蜂花粉及其提取物对酒精性肝损伤的防治效果,利用持续酒精灌胃的方法建立了大鼠酒精性肝损伤模型,测定了血清中AST、ALT、GSH-ST、GSH、TG、MDA水平,并通过光镜观察了肝脏的病理变化。结果显示,酒精性肝损伤可致血清GSH-ST不断消耗使其浓度下降,TG水平不断增高,除了花粉提取物C组外,其他各组均能非常显著地提高血清GSH-ST水平;提取物B、C、D组能显著降低血清TG水平;提取物B、C组显著提高血清GSH水平。各提取物组肝脏脂肪变性和坏死等病理改变明显减轻,尤以C、D、E组为佳。实验结果表明,花粉及其提取物对酒精性肝损伤具有保护作用,提取物C、D、E组分对酒精性肝损伤的保护作用优于花粉。

To investigate the preventive and curative effects of rape bee pollen on alcoholic liver disease （ALD） of rats, ALD models of rats were established by intragastric infusion of 42°liquar. After four weeks, the activities of serum ALT, AST, MDA, GSH-ST, GSH and TG were determined. Meanwhile, the pathological changes of liver were observed under light microscope after HE staining. Compared with that in the control group, the level of serum TG significantly increases, and the content of GSH-ST significantly decreases due to alcoholic injury. The groups of rape bee pollen extract except group C can significantly increase the level of GSH-ST in blood serum. The group of B, Cand D can decrease the level of TG in blood serum. The group of B, and C can increase the level of GSH in blood serum. The results showed that rape bee pollen and its different extracts can reverse the damage caused bv alcohol, esoecially for the group C, D and E.