白首乌多糖的免疫活性研究

Study on Immune Activity of Radix Cynanchi Bungei Polysaccharides

目的:研究了白首乌多糖(RCBPs)体外对兔T淋巴细胞转化及体内对正常小鼠免疫功能的影响。方法:将RCBPs分为2、4、6g/L三个剂量组,分别加入含有兔血的营养瓶内,通过观察T淋巴细胞的转化情况,研究多糖体外免疫功能状况;另将RCBPs分为高、中、低三个剂量组(150、100、50mg/kg·d),连续给小鼠灌胃7d,并进行小鼠体内免疫功能测试。结果:RCBPs各剂量组,均能极显著地(p<0.01)促进PHA诱导的家兔T淋巴细胞在体外的增殖能力;RCBPs能提高小鼠的胸腺和脾脏指数、巨噬细胞的吞噬功能、增强小鼠迟发型超敏反应、加速碳粒的清除。结论:白首乌多糖具有免疫调节活性。

Objective： To study the effects of Radix Cynanchi Bungei polysaccharides （RCBPs） on transformation of T lymphocytes of rabbit phtohemagg luti and immune function in mice. Methods： Three doses of RCBPs （2, 4 and 6 g/L） were respectively put into bottle with Pgytohemagg lutinin （PHA） and rabbit blood. The effect of RCBPs on immunity in vitro was studied by observing transformation of T lymphocytes; The dosages of RCBPs （150, 100, 50 mg/kg · d） were given to mice orally for seven days.The effects on immune function in the mice were investigated. Results： Three doses of RCBPs can significantly promote （p 〈 0.01） the ability of rabbit T lymphocytes. The RCBPs can improve the Thymus and spleen indexes, the celiac macrophage ability of engulfing CRBC, the delayed type hypersensitivity ability and the macrophage-engulfing carbon granula ability. Conclusion： The RCBPs has certain activity of immune regulation.