植物雌激素对MCF-7乳腺癌细胞DNA损伤的影响

Effects of Phytoestrogens on DNA Damage of MCF-7 Breast Cancer Cells

目的:观察植物雌激素三羟异黄酮(Gen)、二羟异黄酮(Dai)、五羟黄酮(Que)和姜黄素(Cur)对MCF-7乳腺癌细胞增殖和DNA损伤的影响。方法:以3、9、27、54μmol/L的植物雌激素与MCF-7细胞共培养24、48或72h,用四唑盐(MTT)试验和单细胞凝胶电泳(SCGE)观察环境雌激素影响细胞增殖和DNA损伤程度。结果:Que和3μmol/L的Gen、Dai、Cur使MCF-7细胞增殖明显增加,但54μmol/L的Gen、Cur却明显抑制细胞的增殖。27、54μmol/L的Gen和Cur使DNA分子迁移长度(CTL)明显增加,且呈剂量-效应和时间-效应关系。Dai、Que对细胞中DNA分子迁移长度无影响。结论:27μmol/L和54μmol/L的Gen和Cur引起MCF-7细胞中DNA分子损伤,抑制细胞增殖。Que和Dai对细胞中DNA分子无损伤,Que和低浓度的Dai促进细胞增殖。

Objective： To observe the effect of phytoestrogens （genistein, Gen, daidzein, Dai, quercetin,Que, and curcumin, Cur） on DNA damage and proliferation of MCF-7 breast cancer cells. Methods： MCF-7 breast cancer cells were cultured with 3,9, 27 and 54 umol/L Gen or Dai or Que or Cur on 24, 48 or 72 h. The proliferation and DNA damage of MCF-7 breast cancer cells were observed by MTT test and single cell gel electrophoresis assay （SCGE）. Results： The proliferation of MCF-7 breast cancer cells significantly increases when they are treated with Que or 3 umol/L Gen or Dai or Cur, but it significantly decreases when the MCF-7 breast cancer cell are treated with 54umol/L Gen or Cur. The comet tail length （CTL） in MCF-7 breast cancer cells significant increases when they are treated with 27 umol/L and 54 umol/L Gen or Cur and show dose-response and timeresponse relationships, but it does not significanly change when the MCF-7 breast cancer cell are treated with Dai or Que. Conclusion： 27 umol/L and 54 u mol/L Gen or Cur can induce DNA damage and inhibits the proliferation of MCF-7 breast cancer cells. Dai orQue does not cause DNA damage and promotes the proliferation of MCF-7 breast cancer cells at the low concentration of Dai.