淡豆豉、黄大豆及黑大豆体内外抗蛋白非酶糖化作用研究

In vitro and in vivo Comparison of Inhibition Effects of Yellow Soybean,Black Soybean and Semen sojae Preparatum on Non-enzyme Glycation

为比较豆类及其发酵品抗蛋白非酶糖化作用,以四氧嘧啶腹腔注射小鼠复制糖尿病模型,正常组和模型组灌胃自来水,黄大豆组、黑大豆组、淡豆豉组(均18.5g生药/kg·d)连续灌胃56d,取血清和主动脉弓测定糖化终末产物。体外试验将牛血清白蛋白(BSA)与D-葡萄糖体外孵育,与此同时分别加入不同浓度的淡豆豉、黄大豆和黑大豆提取物,糖化系统建立90d时,测定各反应体系荧光值。结果表明,各组豆类主动脉糖化终末产物明显降低(p<0.05,p<0.01);除黑大豆组外血清糖化终末产物明显降低(p<0.01)。豆类体外能明显抑制非酶糖化反应的最低浓度在1～100mg/ml之间,且随浓度升高,抑制作用明显。在同等浓度下,淡豆豉的抑制作用强于两种大豆,而两种大豆之间无明显区别。本研究豆类具有抗蛋白非酶糖化作用,其中发酵品淡豆豉作用最强。

To compare the effects of yellow soybean, black soybean and Semen sojae Preparaturn on non-enzyme glycation in vitro and in vivo, diabetes mellitus model of mice was established by intraeritoneal injecting of alloxan, and the mice were given intragastrically with yellow soybean, black soybean group and Semen sojae Preparatum group （18.5 g medicinal material/kg · d） for 56 days, while the normal and model group were treated with water. Subsequently, the levels of serum and aortic advanced glycation end products（AGEs） were assayed. Bovine serum albumin （BSA） and D-glucose were incubated, simultaneously adding yellow soybean, black soybean and Semen sojae Preparatum extracts of different concentrations respectively. The fluorescent values were determined after the glycation systems were established for 90 days. The results showed that the aortic AGEs are low in the above legume groups, and the serum AGEs are low except the black soybean group; The above mentioned groups can inhibit the non-enzyme glycation reaction in vitro, and the lowest concentration is between 1- 100 mg/ml. Semen sojae Preparatum is more effective in inhibiting non-enzyme glycation than yellow soybean and black soybean, and no differences were found between yellow soybean and black soybean group. It was thus concluded that Semen sojae Preparatum can markedly inhibit the reaction of non-enzyme glycation, which might be more useful in health care.