螺旋藻蛋白的胃蛋白酶酶解肽对细胞因子的影响

Effects of Peptides Produced from Spirulina Protein Hydrolyzed by Pepsin on Cytokines

目的:为了探索螺旋藻(Spirulina)蛋白酶解肽对家兔血清中IL-1β、IL-6、IL-12、IFN-γ、TNF-α、IL-1Ra、IL-4、IL-10和TGF-β细胞因子的影响。方法:采用盐析法从螺旋藻中提取可溶性螺旋藻蛋白,以胃蛋白酶水解得到螺旋藻蛋白水解物溶液。将所得水解物以3种方式(口服、腹腔注射、耳静脉注射)对家兔给药,分别在给药3、2、2h后,用ELISA试剂盒测定血清中IL-1β、IL-6、IL-12、IFN-γ、TNF-α、IL-1Ra、IL-4、IL-10,TGF-β细胞因子的浓度。结果:与正常生理盐水组相比,IL-1β、IL-1Ra、IL-4、IL-6、IL-12浓度均下降;IFN-γ、IL-10浓度升高;TGF-β没有明显变化。其中IL-4和TNF-α的腹腔注射,IL-1Ra的腹腔注射、静脉注射,IL-6的腹腔注射、静脉注射及IL-1β的腹腔注射变化相当显著,说明这些水解肽通过肠黏膜系统对细胞因子网络起着重要的调节作用。从给药途径看,腹腔注射的作用效果最为显著,其次是静脉注射,口服灌胃则变化较小。结论:螺旋藻蛋白酶解肽主要是通过肠黏膜发挥作用,设法直接通过肠粘膜途径给药可能是一种最有效的给药方式,具有可开发为药物的发展潜力。

Objective： To explore the effects of peptides produced from Spirulina protein hydrolyzed by pepsin on concentrations of IL-1β, IL-6, IL-12, IFN-y, TNF-α, IL-1Ra, IL-4, IL-10 and TGF-βin rabbit serum. Methods： protein was extracted from Spirulina by salting out method, and then hydrolyzed by pepsin. The hydrolysate was administered to rabbits by gavage, intraperitoneal injection and ear vein injection respectively. At 3, 2 and 2 h after administration respectively, ELISA kit was used to determine the concentration changes of IL-1β, IL-6, IL-12, IFN-y, TNF-α, IL-1Ra, IL- 4, IL-10 and TGF- 1β in rabbit serum. Results： The concentrations of IL-1β, IL-1Ra, IL-4, IL-6 and/L-12 decrease, but the concentrations of TNF-α, IFN-y and IL- 10 increase, and the concentration of TGF- β has no obvious change. In intraperitoneal injection group, concentrations of IL-4, TNF-β, IL- 1Ra （ also in ear vein injection injection group）, IL-6 （ also in ear vein injection injection group） and IL- 1β have significant changes, which show that the peptides play an important regulatory role in the cytokine network by mucosal immune system. The effect of intraperitoneal injection is the most markable, followed by ear vein injection and gavage administration successively. As a whole, the concentrations of most cytokines decrease, but the concentrations of TNF- α, IFN-γ and IL-10 increase. It suggests that the peptides produced from Spirulina protein hydrolyzed by pepsin play an important regulatory role for body immune function by regulating cytokines level. Conclusion： Among the three administration methods, the intraperitoneal injection has the most obvious effects, because direct administration to intestinal mucosal is possibly the most effective way. So the peptides produced from Spirulina protein hydrolyzed by pepsin have the promise of drug development.