富锗大麦苗对小鼠免疫功能的调节

Regulation of Germanium-enriched Barley Seedling on Immune Function of Mice

目的:通过测定小鼠细胞免疫与体液免疫,来研究富锗大麦苗对小鼠免疫功能的调节作用。方法:用不同剂量富锗大麦苗对小鼠灌胃,测定小鼠腹腔巨噬细胞吞噬功能,白细胞总数与分类,抗体效价,抗体生成细胞数量以及免疫器官重量等指标,并对其进行统计分析。结果:富锗大麦苗低、中、高剂量均能显著提高小鼠腹腔巨噬细胞的吞噬能力(p<0.01),显著提高小鼠白细胞总数(p<0.05);中、高剂量能明显提高抗鸡红细胞抗体的效价(p<0.01),明显提高抗体生成细胞的数量(p<0.01);低、中剂量明显提高脾脏指数(p<0.01),低剂量有提高肝脏指数的作用(p<0.05)。结论:富锗大麦苗有显著提高小鼠细胞与体液免疫的功能,并与富锗大麦苗剂量呈正相关;富锗大麦苗还能提高免疫器官的重量,并与其剂量有一定相关性。

Objective： To observe the regulation of germanium-enriched barley seedling （GBSE）on the immune function of mice by detecting the cellular immunity and humoral immunity. Methods： After given different dose of GEBS by gavage, a series of items were detected on mice, including phagocytosis test of macrophage on abdominal cavity, quantity and classification of white blood cell （WBC）, titer of antibody, quantity of antibody forming cell and weights of immune organ. Results： Three doses of GEBS all can obviously increase phagocytic function of macrophages （p 〈 0.01） and increase the quantity of WBC （p 〈 0.05）; The medium dose and high dose increase the titer of anti-CRBC antibody （p 〈0.01） and the quantity of antibody-forming cell （p〈0.01） obviously; The low dose and medium dose increase spleen index （p〈0.01） and the low dose group increase liver index （p 〈0.05）. Conclusion： GEBS can improve cellular immunity and humoral immunity of mice obviously, which is positively related to the dose of GEBS; GEBS can also increase the weight of immune organ, and there is certain correlationship between the weight gain and the dose of EGBS.