磁性载体固定化机油降解菌FZ5的研究

Study on immobilizing of lubricating oil-degradation strains FZ5 by magnetic carriers

利用聚乙烯醇-海藻酸钠-磁性竹炭复合制备磁性悬浮小球,该小球的磁矫顽力为28.6 mT,具有较强的磁场,可用于磁场引导分离。利用磁性悬浮小球作为载体对机油降解菌FZ5进行固定化,研究了固定化时间、降解时间、固定化小球投加量、初始pH值和初始机油浓度对机油降油率的影响。结果表明,在固定化挂膜3 d,固定化小球投加量为8%,初始pH值为7,初始机油浓度为400 mg/L,降解培养3 d后对机油的降解率可达91.21%。

Magnetic suspension minor balls were prepared by using sodium alginate, polyvinyl alcohol and magnetic carbon as complex supports. With magnetic coercivity of 28.6 mT, the minor balls have stronger magnetic field and can be used to guide the magnetic separation. The lubricating oil-degradation strains FZ5 was immobilized by magnetic suspension minor balls. The impacts of the immobilizing time, the degradation time, the dosage of immobilizing balls, the initial pH value, and the initial concentration of lubricating oil on the lubricating oil-degradation rate were studied. The results shows that when the immobilizing time was 3 d, the dosage of the immobilizing balls was about 8 percent, the initial pH value was 7 and the initial concentration of lubricating oil was 400 mg/L, the oil-degradation rate reached 91.21% after 3 day＇s degrading cultivation.