脑血管疾病患者体液中SOD、MDA含量测定

Determination of SOD and MDA in Cerebrovascular Disease

本文对67例脑卒中（脑梗塞42例、脑出血25例）病人血清、红细胞、脑脊液、尿进行了超氧化物歧化酶（SOD）、丙二醛（MDA）的含量测定。以34名正常人相同样品作对照。结果：脑卒中患者各标本中SOD的含量显著低于对照组（P＜0．05～0．01），MDA含量显著高于对照组（P＜0．05～0．01）；脑卒中急性期SOD含量低于恢复期，MDA含量高于恢复期，具有显著差异（P＜0．05）；且脑出血患者血清、红细胞SOD含量显著低于脑梗塞患者，而脑脊液SOD含量显著高于脑梗塞，且各标本中MDA的含量也显著高于脑梗塞（P＜0．05）；19例死亡者各标本中的SOD、MDA含量与42例存活者有极显著差异（P＜0.01）；26例患者抗自由基治疗后，其血清、红细胞、脑脊液、尿液SOD含量显著高于未经抗自由基治疗者，MDA显著低于未经抗自由基治疗者。检测结果说明，脂质过氧化及自由基损伤在脑卒中的发病机制中起着重要作用，放对脑卒中患者血清、红细胞、脑脊液、尿液等各标本同时进行SOD、MDA的含量测定，在其发病机理、鉴别诊断＼治疗及预后等各方面具有一定的临床意义。

We measured the superoxide dismutase (SOD) and malondialdehyde (MDA)levels in serum, RBC, CSF and urine of 67 cerebral apoplexy patients (42 cerebral infarction patients, 25 cerebral haemorrhage patients) and compared them with 34 normal subjects.Results: The normal SOD values in serum, RBC, CSF and urine were significantly higher than those of cerebral apoplexy patients (P < 0. 05 ～ 0. 001 ). The normal MDA values in serum,RBC, CSF and urine were significantly lower than those of cerebral apoplexy patients (P< 0. 05～ 0. 01 ). In acute stage of cerebral apoplexy, SOD values were significantly lower and the MDA values were significantly higher than those in the stationary phase (P < 0. 05). The SOD values in serum and RBC of cerebral haemorrhage patients were significantly lower than those of cerebral infarction patients, and the MDA values were significantly higher than those of cerebral infarction patients (P< 0. 05). The SOD and MDA values between the died (19 cases) and the living patients (42 cases) were obviously different (P < 0. 01 ). In serum RBC, CSF and urine of the patients (26 cases) treated with anti - free radical therapy and routine therapy, the SOD values were significantly higher and the MDA were significantly lower than those of the patients treated with routine therapy only. The experimental results showed that lipid peroxidation and free radical damage played an important role in pathogenesis of cerebral apoplexy. So, tO simultaneously determine the SOD and MDA values in serum RBC, CSF and urine of cerebral apoplexy patients,had useful clinic significance on pathogenesis, differential diaignosis, therapy and prognosis, etc.