摘要
用新型除草剂Oryzalin作为彩色马蹄莲体细胞染色体加倍的诱导剂,以愈伤组织芽点为处理材料,研究了不同处理浓度和处理时间的除草剂对彩色马蹄莲染色体加倍的影响。结果表明:以0.001 6%的浓度处理36 h效果最好,变异率达34%。本研究为培育彩色马蹄莲四倍体新品种,以及进一步检测四倍体植株能否克服马蹄莲种间杂交障碍提供种质资源储备。
With the new herbicide-oryzalin to treat the callus with young buds of coloured Zantedeschia aethiopica in vitro, the effects of oryzalin of different concentration and time duration on doubling chromosome of coloured Z. aethiopica were studied. The result showed that 0. 001 6 % with 36 h was the best one to induce mutation of eoloured Z. aethiopica, and the mutation rate was up to 34%. The resuits can provide genetics back up for breeding tetraploid new variety and see if tetraploid plants can overcome the obstacle of interspecifie cross of coloured Z. aethiopica.
出处
《云南农业大学学报(自然科学版)》
CAS
CSCD
2008年第6期806-810,共5页
Journal of Yunnan Agricultural University:Natural Science
基金
云南农业大学青年基金(A2002002)