镰刀菌诱导后苦瓜幼叶cDNA文库的构建和鉴定

Construction and Identification of Young Leaf cDNA Library from Momordica charantia L.Infected with Fusarium solani

以白粉病菌侵染的苦瓜幼叶为材料，以λTrip IEx2为载体，利用SMART技术构建了1个由镰刀菌病原诱导后的苦瓜幼叶cDNA文库．结果显示，初始文库的滴度为4．0×10^6pfu·mL^-1，扩增后滴度为8．1×10^9pfu·mL^-1，插入片段大小分布在500～3000bp，平均值大于1000bp，重组效率为98％．

To clone the powdery mildew resistance related gene from bitter melon （Momordica charantia L. ） , a cDNA library with cDNAs of bitter melon leaves infected with Fusarium solani was constructed by SMART （switching mechanism at 5＇ end of RNA transcript） and LD-PCR （long distance PCR） techniques. Results showed that the titers of unamplified and amplified cDNA library were 4.0 × 10^6 pfu . mL^-1 and 8.1 × 10^9 pfu . mL^-1 respectively, and the length of inserted fragments was 500-3000 bp,with an average length of more than 1 000 kb,and the recombination rate of 98%.