摘要
以白粉病菌侵染的苦瓜幼叶为材料,以λTrip IEx2为载体,利用SMART技术构建了1个由镰刀菌病原诱导后的苦瓜幼叶cDNA文库.结果显示,初始文库的滴度为4.0×10^6pfu·mL^-1,扩增后滴度为8.1×10^9pfu·mL^-1,插入片段大小分布在500~3000bp,平均值大于1000bp,重组效率为98%.
To clone the powdery mildew resistance related gene from bitter melon (Momordica charantia L. ) , a cDNA library with cDNAs of bitter melon leaves infected with Fusarium solani was constructed by SMART (switching mechanism at 5' end of RNA transcript) and LD-PCR (long distance PCR) techniques. Results showed that the titers of unamplified and amplified cDNA library were 4.0 × 10^6 pfu . mL^-1 and 8.1 × 10^9 pfu . mL^-1 respectively, and the length of inserted fragments was 500-3000 bp,with an average length of more than 1 000 kb,and the recombination rate of 98%.
出处
《河南农业大学学报》
CAS
CSCD
北大核心
2008年第5期487-490,共4页
Journal of Henan Agricultural University
基金
河南农业大学博士研究基金项目(30200274)
关键词
苦瓜
镰刀菌
CDNA文库
构建
鉴定
Momordica charantia L.
Fusarium solani
cDNA library
construction
identification