水通道蛋白3在四种皮肤肿瘤中的表达

Expression of aquaporin 3 in four cutaneous tumors

目的探讨水通道蛋白3（AQP3）在四种皮肤肿瘤组织中的表达及意义。方法应用免疫组织化学方法检测30例脂溢性角化病、15例Bowen病、32例鳞状细胞癌、17例恶性黑素瘤及15例正常人皮肤组织中AQP3的表达。结果脂溢性角化病、Bowen病、鳞状细胞癌、恶性黑素瘤及正常人表皮组织中均存在AQP3蛋白的表达；脂溢性角化病皮损中AQP3表达水平与正常人对照组差异无统计学意义（P〉0．05）；Bowen病、鳞状细胞癌及恶性黑素瘤皮损中AQP3蛋白表达显著高于正常人对照组（P〈0．01），其中鳞状细胞癌与恶性黑素瘤的表达最强，均显著高于Bowen病（P〈0．01），但鳞状细胞癌与恶性黑素瘤比较差异无统计学意义（P〉0．05）。此外，在鳞状细胞癌中AQP3的表达与肿瘤的分化有显著相关性（P〈0．01）；在已转移恶性黑素瘤中AQP3的表达显著高于未转移恶性黑素瘤（P〈0．05）。结论AQP3在皮肤恶性肿瘤中表达上调。

Objective To investigate the expression of aquaporin 3 （AQP3） in lesions of four cutaneous tumors. Methods Immunohistochemistry was utilized to measure the expression of AQP3 in tissue samples from 30 patients with seborrheic keratosis, 15 patients with Bowen＇s disease, 32 patients with squamous cell carcinoma, 17 patients with malignant melanoma and 15 normal human controls. Results AQP3 was observed in all the tissue samples from both patients and controls. A significant increment was noticed in the expression of AQP3 in patients with Bowen＇s disease, squamous cell carcinoma and malignant melanoma compared with the normal controls （all P 〈 0.01 ）, while no significant difference was found between patients with seborrheic keratosis and the controls （P 〉 0.05）. The highest expression of AQP3 was obtained in lesions from patients with squamous cell carcinoma and malignant melanoma, followed by those with Bowen＇s disease （both P 〈 0.01 ）, and there was no significant difference between squamous cell carcinoma and malignant melanoma （P 〉 0.05 ）. The differentiation status of squamous cell carcinoma significantly correlated with the expression of AQP3 （P 〈 0.01 ）. AQP3 was significantly higher in malignant melanoma with metastasis than that in malignant melanoma without metastasis （P 〈 0.05 ）. Conclusion The expression of AQP3 is upregulated in malignant skin tumors.