霉酚酸酯对糖尿病大鼠肾组织早期炎症反应的影响

Effect of mycophenolate mofetil on the expression of early inflammatory reaction in diabetic rats

目的:探讨霉酚酸酯(MMF)对链脲佐菌素(STZ)诱导的糖尿病大鼠肾组织早期炎症反应的影响。方法:3 6只雄性SD大鼠随机分为正常对照组、糖尿病模型组和MMF治疗组,于4周、14周末每组分别处死6只大鼠,处死前进行2 4 h尿蛋白定量检测,处死后免疫组织化学染色观察调节激活正常T细胞表达和分泌的细胞分子(RANTES)、单核/巨噬细胞表面特异性标志抗原(ED-1)、Ⅳ型胶原蛋白(ColⅣ-)在肾组织中的表达,RT-PCR方法观察RANTES mRNA在肾组织中的表达。结果:MMF可显著抑制大鼠2 4 h尿总蛋白的排泄,下调肾组织RANTES,ED-1,ColⅣ-蛋白及RANTES mRNA的表达。结论:早期应用MMF对糖尿病大鼠的肾保护作用可能与抑制肾脏早期炎症反应有关。

Objective To investigate the effect of mycophenolate mofetil （ MMF ） on early inflammatory reaction of renal lesion in streptozotocin （ STZ ） -induced diabetic rats. Methods Thirtysix male Sprague-Dawley rats were randomly divided into 3 groups after uninephrectomy： normal control group, diabetic model group, and MMF-treated group. Six rats in each group were sacrificed at the 4th week and 14th week after STZ injection. Twenty-four hour urinary protein （24 h Upro） count was measured before death. The expressions of regulated on activation of normal T expressed and secreted （RANTES）, ectodermal dysplasia （ED-1）and Col-Ⅳ protein in the renal tissue were detected by immunohistochemistry. The expression of RANTES mRNA in the renal tissue was detected by RT-PCR. Results MMF prevented the increasing of 24h Upro in diabetic rats, and the expressions of RANTES, ED-1 , Col-Ⅳ protein and RANTES mRNA in the kidney of MMF-treated rats were significantly decreased. Conclusion MMF plays an early renal protective role in diabetic nephropapossibly through inhibition of early inflammatory reaction.