C57BL/6J小鼠EAE模型的建立及巨噬细胞移动抑制因子的表达

Mouse model of experimental antoimmune encephalomyelitisin C57BL/6J and expression of macrophage migration inhibitory factor

目的：通过观察巨噬细胞移动抑制因子（macrophage migration inhibitory factor,MIF）在慢性实验性变态反应性脑脊髓炎（experimental autoimmune encephalomyelitis,EAE）小鼠脑和脊髓中的动态表达变化,探讨MIF与EAE发病的关系。方法：72只无特定病原体的6-8周健康雌性C57BL/6J小鼠随机分为EAE组、空白组和佐剂组。用mMOG35-55和完全弗氏佐剂（complete Freund’s adjuvant,CFA）免疫小鼠制成慢性EAE动物模型,免疫组织化学技术检测不同组别小鼠脑和脊髓中MIF的表达变化。结果：EAE组小鼠在疾病各个时期,中枢神经系统（CNS）中均可见MIF表达升高,各个时期MIF表达与空白组和佐剂组相比均有统计学意义（P〈0.05）;在EAE组,MIF在疾病发病高峰期表达最高,与其他时间点相比差异有统计学意义（P〈0.05）。结论：MIF在EAE小鼠发病过程中有明显表达,可能与EAE的发病和恶化相关。

Objective To detect the expression of macrophage migration inhibitory factor （MIF） in the brain and spinal cord of chronic non-remitting model of experimental autoimmune encephalomyelitis （EAE） mouse, and to discuss their effect on EAE/MS. Methods Seventy-two female SPF C57BL/6J mice, aged 6 -8 weeks, were randomly divided into an EAE group, a blank group, and an adjuvant group. The mice were immuned by mMOG35.55 and CFA. Immunohistochemic technique was used to detect the expression of MIF in the brain and spinal cord. Results In the EAE group, we observed up-regulation of MIF of central nervous system （CNS） at onset, peak and chronic phase. During each phase, the difference of MIF between the EAE group and each of the other 2 groups was significant （ P 〈 0.05 ）. In the EAE group, the expression of MIF was the highest at the peak, which was different from other periods （ P 〈 0.05 ）. Conclusion MIF significanty expressed during the procedure of EAE disease, and may be related with the onset and exacerbation of EAE.