NGX6基因在肝细胞癌中的表达及意义

Expression and significance of NGX6 gene in human hepatocellular carcinoma

目的:探讨NGX6基因在肝细胞癌及癌旁组织中有无表达及其与临床病理特征的关系。方法:肝细胞癌患者组织标本分为癌与癌旁对照组,应用RT-PCR检测45例肝细胞癌及配对癌旁组织NGX6表达,并对其表达和各临床病理参数的关系进行分析。结果:癌组织NGX6阳性表达率35.5%(16/45),NGX6表达强度相对值为0.245±0.060;癌旁组织阳性表达率为77.8%(35/45),强度相对值为0.352±0.113;癌与癌旁组织的表达阳性率及强度比较差异有统计学意义(P<0.05);肝细胞癌组织NGX6表达与TNM分期(χ2=6.106,P=0.042)、淋巴结转移(χ2=5.237,P=0.022)有关。结论:NGX6基因转录水平的表达降低或缺失,可能在原发性肝细胞癌的发生过程中起了重要作用,NGX6基因表达水平有可能作为一项早期预测肝癌发生发展的指标。

Objective To examine the expression of NGX6 gene and to investigate its association with the clinico-pathological characteristics in hepatocellular carcinoma （ HCC ）. Methods Samples from 45 patients were divided into the hepatocellular carcinoma tissue group and the matched paracancerous tissue group. Reverse transcription polymerase chain reaction（RT-PCR） was used to detect the expression of NGX6 gene in the hepatocellular carcinoma and the surrounding normal tissue specimens. Results The positive rates of NGX6 in the hepatocellular carcinoma and the matched paracancerous tissues were 35. 5% （ 16/45 ） and 77. 8% （ 35/45 ） , and the ratios of NGX6/ G3PDH mRNA were 0. 245 ±0. 060 and 0. 352 ±0. 113. There was significant difference in the 2 groups （P 〈 0. 05 ）. The expression of NGX6 gene was related to TNM staging （X^2 = 6. 106, P =0. 042 ） and lymph node metastasis （ X^2 = 5. 237, P = 0. 022 ） in hepatocellular carcinoma. Conclusion There is positive expression of NGX6 in the hepatocellular carcinoma and the matched paracancerous tissues. The low-expression or non-expression of NGX6 gene plays an important role in the gene transcription level in hepatocellular carcinoma. The expression of NGX6 gene is related with TNM staging and lymph node metastasis in hepatocellular carcinoma. Expression of NGX6 might be used as an early indicator of the occurrence and development of hepatocellular carcinoma.