摘要
目的比较PreS1、HBV-DNA、HBeAg反映病毒复制的临床应用价值。方法对疑有HBV病毒复制的乙型肝炎患者血清标本分别采用实时荧光定量PCR检测HBV-DNA、时间分辨荧光免疫分析技术(TRFIA)定量检测HBV-e抗原和HBV PreS1抗原。结果PreS1抗原阳性率87.5%(460/526)高于HBV-DNA71.1%(374/526),有显著性差异(χ2=46.3,P<0.001),PreS1抗原阳性率高于HBeAg 37.1%(195/526),有显著性差异(χ2=251.6,P<0.001),HBV-DNA阳性率高于HBeAg(χ2=161.8,P<0.001),有显著性差异。结论TRFIA方法定量检测PreS1抗原,灵敏度高,特异性强,能够准确及时敏感地反映患者体内乙型肝炎病毒的复制情况。
Objective To compare the clinical significances of serum PreS1 , HBV-DNA and HBeAg levels for virus replication. Methods Five hundred and twenty-six serum specimens of hepatitis B patients were collected to test serum HBV-DNA by fluorescent quantitation PCR and the PreS1, HBeAg levels by Time-resolved fluorescent immuno assay (TRFIA). Results The positive rate of PreS1 was higher than that of HBV-DNA ( X^2 = 46.3, P 〈 0. 001 ) which was higher than that of HBeAg( X^2 = 161.8 ,P 〈 0. 001 ). Conclusion Detection of PreS1 by TRFIA has better the sensitivity and specificity and probably has accurately reflected the hepatitis B virus replication in patients.
出处
《胃肠病学和肝病学杂志》
CAS
2008年第11期907-909,共3页
Chinese Journal of Gastroenterology and Hepatology
基金
南京医科大学科技发展基金项目(06NMUM072)
无锡市卫生系统指令性科研项目(XM0710)
关键词
时间分辨荧光免疫测定
乙型肝炎标志物
乙肝病毒前S1抗原
Time-resolved fluorescent immunoassay
The marker of hepatitis B surface antigens
Hepatitis B virus PreS1