Syndecan-1在结直肠腺瘤和腺癌中的表达变化及其意义

Expression of Syndecan-1 in Colorectal Adenoma and Adenocarcinoma and its Significance

背景:Syndecan-1是一种由硫酸乙酰肝素链和硫酸软骨素链修饰的Ⅰ型跨膜蛋白多糖,主要表达于上皮细胞表面,与肿瘤的多种生物学行为关系密切。目的:研究Syndecan-1在结直肠腺瘤和腺癌中的表达,探讨其与结直肠腺瘤和腺癌发生的关系。方法:分别采用逆转录聚合酶链反应(RT-PCR)和免疫组化染色检测56例结直肠腺瘤、42例腺癌和20例正常结肠组织中Syndecan-1mRNA和蛋白表达,并分析其与结直肠腺癌临床病理特征的关系。结果:RT-PCR和免疫组化染色结果显示Syndecan-1mRNA和蛋白在重度异型增生腺瘤和腺癌中的表达显著低于正常结肠组织和轻、中度异型增生腺瘤(P<0.05),正常结肠组织与轻、中度异型增生腺瘤之间以及重度异型增生腺瘤与腺癌之间其表达无明显差异。Syndecan-1mRNA和蛋白表达与腺癌分化程度、浸润深度、淋巴结转移、远处转移以及TNM分期相关(P<0.05),与性别、年龄以及是否合并结直肠腺瘤无关。结论:随着结直肠腺癌恶性程度的增加,Syndecan-1表达显著降低。Syndecan-1介导的细胞间黏附破坏在结直肠癌的致癌机制中可能起关键作用。

Syndecan-1 is a type I transmembrane proteoglycan modified by heparan sulfate and chondroitic acid. It is mainly expressed on the surface of epithelial cells, and is closely related to many biological behaviors of cancer. Aims： To investigate the expression of Syndecan-1 in colorectal adenoma and adenocarcinoma, and to explore its relationship with the progression of colorectal adenoma and adenocarcinoma. Methods： Expressions of Syndecan-1 mRNA and protein in 56 colorectal adenomas, 42 adenocarcinomas and 20 normal colonic tissues were determined by reverse transcriptase polymerase chain reaction （RT-PCR） and immunohistochemistry, respectively. Relationship between expression of Syndecan-1 and clinicopathological characteristics of colorectal adenocarcinoma was analyzed. Results： RT- PCR and immunohistochemistry showed that expressions of Syndecan-1 mRNA and protein in high degree dysplastic adenoma and adenocarcinoma were significantly decreased than those in normal colonic tissues, and low and moderate degree dysplastic adenoma （P〈0.05）. No significant differences were found in low degree dysplastic adenoma, moderate degree dysplastic adenoma and normal colonic tissues. And there was also no significant difference between high degree dysplastic adenoma and adenocarcinoma. The expressions of Syndecan-1 mRNA and protein in adenocarcinoma were correlated with degree of differentiation, depth of infiltration, lymph node metastasis, distant metastasis and TNM staging （P〈0.05）, but not with gender, age and with or without colorectal adenoma. Conclusions： With the increase of malignant degree of colorectal adenocarcinoma, the expression of Syndecan-1 decreases obviously, which indicates that the destruction of intercellular adherence mediated by Syndecan-1 may play a key role in carcinogenesis of colorectal cancer.