摘要
建立甘薯叶片中有效成分叶黄素含量的高效液相色谱测定方法,以寻求含量高的甘薯品种。采用Waters SunFireTM C18(150mm×4.6mm,5μm)色谱柱;以甲醇—水为95:5(V/V)为流动相,流速为0.8mL.min-1;检测波长为445nm;外标法定量。此色谱条件下,叶黄素含量在5～100μg.mL-1范围内时,与峰面积呈线性关系;样品平均回收率为99.5%;相对标准偏差(RSD)为1.9%(n=5)。该方法灵敏、准确、专属性强,适用于甘薯叶片中叶黄素的测定;大部分供试品种间存在显著差异,其中以苏薯8号的含量最高。
A HPLC method for the determination of lutein in leaves of sweet potato was developed in this research for selecting the breed which has the highest content of lutein. The analysis was carried on a Waters SunFireTM C18 （150mm×4.6mm,5μm） column. The mobile phase was methanol-water （95： 5, v/v） , the flow-rate was 0.8 mL·min^- 1 and wave-length was 445 nm. Linearity was found in the range of 5 - 100 μg·mL^-1 , the average recovery was 99.5% , and the relative standard deviations for lutein was 1.9% （ n = 5 ）. This method is reliable, accurate and suitable for the determination of lutein in leaves of sweet potato. There were significant differences in the lutein content of most samples tes[ed and among them, Sushu No. 8 are the highest.
出处
《植物研究》
CAS
CSCD
北大核心
2008年第6期767-769,774,共4页
Bulletin of Botanical Research
基金
国家教育留学回国科研启动基金(教外司留[2004]527号)
农业部寒地作物生理生态重点开放实验室专项基金
