子宫颈病变脱落细胞中端粒酶的表达及临床意义

Expression and Clinical Significance of hTERC Gene in Cervical Lesion

目的探讨端粒酶基因在不同程度子宫颈病变脱落细胞中的表达及临床意义。方法采用荧光原位杂交方法,检测15例正常子宫颈脱落细胞、76例子宫颈上皮内瘤变及27例浸润性子宫颈癌脱落细胞hTERC的表达情况。结果hTERC基因在正常子宫颈脱落细胞、CINⅠ、CINⅡ、CINⅢ及浸润性子宫颈癌中的阳性表达率分别为6.7%、18.8%、52.0%、71.4%及92.5%。hTERC基因表达子宫颈癌组与CIN各组及正常对照组比较均有显著性差异(P均<0.05),CINⅡ∽Ⅲ组与正常对照组比较有非常显著性差异(P<0.01)。结论hTERC基因高表达在宫颈癌发生发展中可能起重要作用,hTERC基因检测有望成为子宫颈癌早期筛查方法之一。

Objective To investigate the expression and clinical significance of the hTERC gene in cervical lesions.Methods The expression of hTERC gene in tissues from 27 infiltrating cervical cancer,76 cervical intraepithelial neoplasia and 15 cnormal control cervix were detected by the fluorescent in situ hybridization（FISH）.Results Positive expression rates of the hTERC gene in normal cervixes、CINⅠ、CINⅡ、CINⅢ and infiltrating cervical cancer were 6.7%、18.8% 、52.0%、71.4% and 92.5%.Compared with control cervix and CIN,hTERC gene positive expression in infiltrating cervical cancer was different significantly（P〈0.05）,and there was a significantly difference between CINⅡ～CINⅢ and control cervix（P〈0.01）.Conclusion Our study suggested that the over expression of hTERC gene may play an important role in cervical carcinogenesis,therefore it can be used as a better tumor index for early cervical cancer screening and accessory diagnosis.