鼻咽癌中自发凋亡与临床放射敏感性的研究

Relationship among Spontaneous Apoptosis Index and Clinical Radiation Sensitivity in Nasopharyngeal Carcinoma

目的探讨鼻咽癌组织中细胞自发凋亡在预测临床放射敏感性中的意义。方法对38例初治、无远处转移、放疗前鼻咽癌患者活检标本,采用TUNEL法检测细胞自发凋亡,临床观测患者放疗过程中鼻咽部肿瘤的消退情况。结果自发凋亡率与鼻咽癌瘤体的消退率呈显著正相关(放疗剂量为40Gy时,γ=0.836;放疗剂量为70Gy时,γ=0.909;P<0.01)。患者性别、年龄及分期与鼻咽癌瘤体的消退率均无相关性(P>0.05)。结论鼻咽癌中细胞自发凋亡率可以作为鼻咽癌临床放射敏感性可能的预测指标。

Objective To investigate the spontaneous apoptosis index（SAI）in the prediction of clinical radiation sensitivity of nasopharyngeal carcinoma.Methods 38 patients with nasopharyngeal carcinoma receiving irradiation therapy alone without distant metastasis were registered in this study.Specimens were obtained before irradiation.The apoptotic index（AI）of each tissue specimen was evaluated by terminal deoxynucleotidy transferase mediated deoxyuridine triphophate nick-labling（TUNEL）assay.The AI was then correlated to the clinical response to radical radiotherapy.Results There was a significantly positive correlation between SAI and the rate of tumor regression（40 Gy,γ=0.836;70 Gy,γ=0.909;P〈0.01）.Sex,age and clinic stage showed no significant corre-lation with tumor response.Conclusion SAI could predict the clinical radiosensitivity of nasopharyngeal carcinoma.