糖皮质激素对戊四氮致痫大鼠脑内一氧化氮合酶表达的影响

Effect of glucocorticoids on expression of nitric oxide synthase in cerebral cortex of epileptic rats induced by pentylenetetrazole

目的探讨糖皮质激素(GC)对致痫大鼠大脑皮质脑内一氧化氮合酶(NOS)表达的影响。方法本实验用免疫印迹技术和图像分析技术观察了经GC预处理后致痫大鼠行为学的改变与脑内NOS表达的变化。结果戊四氮组大脑皮质内NOS含量明显高于对照组,戊四氮+GC组和GC组大脑皮质内NOS含量明显低于对照组,二者均有显著性差异(P<0.05)。结论糖皮质激素具有明显的抑制NOS表达的作用和抗痫效应。

Objective To investigate the effect of glucocorticoids on expression of nitric oxide synthase （NOS） in cerebral cortex of epileptic rats. Methods The rat model of epilepsy was induced by pentylenetetrazole and the expression of NOS in cerebral cortex was detected by western blotting. Results The expression of NOS in cerebral cortex of epileptic rats was increased in pentylenetetrazole group, and there was significant difference （ P 〈 0.05 ） in comparison with that of control group. Compared with pentylenetetrazole group, the expression of NOS in cerebral cortex （ P 〈 0.05 ） could be significantly decreased by glucocortieoids. Conclusion Glucoeorticoids have inhibitory effect on the expression of nitric oxide synthase in cerebral cortex of epileptic rats.