近红外光谱用于卷柏属药用植物种下类型鉴别及种间关系探讨

Discussion on identification and phylogenetic relationships of medicinal plants in Selaginellaceae by NIRS

目的:研究解决卷柏属植物药分类鉴定中的难点问题,探讨该属药用种间亲缘关系。方法:应用傅立叶变换近红外光谱仪、固体光纤探头、InGaAs检测器,扫描范围4000-9000cm-1,每份样品扫描32次,并重复测定5次求平均值。平均光谱经处理后转换成一阶导数光谱,再用OPUS/INDENT软件进行聚类分析。并获取2类薄叶卷柏样品的二阶导数光谱。结果:根据光谱图及聚类图,薄叶卷柏和异穗卷柏各2个类型均各为同种,且薄叶卷柏变异类型应视为变种。垫状卷柏与卷柏亲缘关系密切,同作卷柏药用合理。江南卷柏与薄叶卷柏关系较近,民间同作一药有合理性。结论:近红外光谱可简单快速地用于解决药用植物种下分类鉴定的一些疑点问题,可推断某些种间亲缘关系。

Objective： to study and solve some difficult problems in identification of medicinal plants in Selaginellaceae, and research the phylogenetic relationships among them. Methods： All the spectra were collected by using a Fourier transform near-infrared spectrometer equipped with a fiber-optic probe and a InGaAs detector for reflectance measurements. All the samples were scanned from 9 000 cm-~ to 4 000 cm-~, and each sample spectrum was obtained as an automatic mean of 32 times. The samples were measured 5 times. The average spectra obtained were transformed to first-order derivative spectra, and the latter were computerized with OPUS/INDENT operation software to conduct cluster analysis. Results： According to the information obtained from spectrograms and dendrograms, two types of S. heterostachys and two types of S. delicatula were. identified as the same species respectively, and the variable type of the latter should be treated as a variety. S. tamariscina and S. pulvinata have very close relationship, indicating it is reasonable that they were applied as the same medicinal materials Juanbai. There is close phylogenetic relationship between S. moellendorffii and S. delicatula .This is also accord with the fact that they were used as the same folk herbal medicine. Conclusion： It shows that NIRS can be conveniently and quickly used for authentication of the medicinal plant species, resolving some correlative difficult problems, and inducing certain relationships between interspecies.