芪丹煎剂有效成分提取方法优选研究

Study on better extraction method for Qidan decoction

目的:研究芪丹煎剂有效成分超声法提取的最适条件。方法:利用正交试验设计,对水量、提取时间和提取次数进行分析,找出最适条件。按最适条件重复3次,考察重复性。结果:水量为14倍、提取时间为30min,提取次数为3次时有效成分的总含量最大,3次平行试验有效成分总含量RSD为0.86%。结论:超声法提取的最佳条件为:水量14倍、提取时间30min,提取次数3次。本方法重复性较好。

Objective： To study the better extraction method for Qidan decoction. Method： Orthogonal design was used to find befitting conditions. The water quantity, extraction time and frequency were selected as observational conditions. The optimum conditions were repeated three times to study repetition. Results： The optimum extracting procedure is 14 times water, extracting 3 times ,the time was 30 min each time. The active ingredient content RSD of three parallel trial is 0.86%. Conclusion： The optimal condition for ultrasonic extraction is 14 times water, extracting 3 times, 30 min per time. This method is of good repeatability.