赤芍药提取物对兔颈动脉介入术后增殖内膜相关基因表达的影响

Effect of Radix Paeoniae Bubra on NADPH oxidase p22phox,MCP-1 mRNA expression and neointimal hyperplasia after balloon injury in cholesterol-fed rabbits

目的:探讨赤芍药提取物对高脂喂养兔颈动脉球囊损伤后血管内膜增殖与还原型烟酰胺腺嘌呤二核苷酸磷酸(NADPH)氧化酶亚单位p22phox及单核细胞趋化蛋白-1(MCP-1)的影响。方法:采用Clowes方法建立兔颈总动脉球囊损伤模型,并给予赤芍药提取物。免疫组化染色测定兔颈动脉新生内膜成分、增殖细胞核抗原(proliferat ingcellnuclearantigen,PCNA)及巨噬细胞表达,血管特殊染色观察1型胶原增生,图像分析系统测定新生内膜面积;原位杂交染色及逆转录聚合酶链反应检测增殖血管内膜的NADPH Oxidase p22phox及MCP-1 mRNA水平。结果:赤芍药提取物可减少PCNA阳性细胞数,抑制型胶原增生,减轻新生内膜形成;显著降低NADPH Oxidase p22phox及MCP-1mRNA表达。结论:赤芍药抑制血管内膜增殖程度,其机制可能与抗氧化应激及抗血管内皮炎症反应有关。

Objective： To observe the effect of Radix Paeoniae Bubra（RPB） on NADPH oxidase p22phox,monocyte chemotactic protein-1 （MCP-1） mRNA expression and neointimal hyperplasia after carotid artery balloon injury in cholesterol-fed rabbits. Methods： The rabbit model of carotid balloon injury was established adopting Clowes method, and treated with extract of RPB. Component of neointima and expression of proliferating cell nuclear antigen （PCNA） and macrophage was determined by immunochemical stain. The collagen of type I was detected by special staining for blood vessels and the area of neointima was measured by image assay system. Expression of NADPH oxidase p22phox mRNA,MCP-1 mRNA was detected by in situ hybridization and transcription-polymerase chain reaction. Results： RPB can attenuate the neointima area and proliferation of collagen type Ⅰ induced by balloon-injury, remarkable prevent the formation of restenosi, and down-regulate expression of NADPH oxidase p22 and MCP-1mRNA significantly and decrease the degree of macrophages infiltration especially in vessel wall of injuring carotid artery. Conclusions： RPB inhibited NADPH oxidase P22Phox and MCP-1 mRNA expression, and modestly reduced neointimal hyperplasia, which might be partly attributed to its antioxidant and inflammatory effects.