五个野生木耳属菌株的ISSR分析

Molecular Identification of Five Wild-type Auricularia Strains Using Inter-simple Sequence Repeat (ISSR) Markers

以7个栽培菌株为参照，采用ISSR分子标记技术对5个野生木耳属菌株进行分析，使用NTSYSpc2．1生物软件对12个供试菌株进行聚类分析，构建系统树。试验筛选出了11个扩增谱带清晰、多态性好的ISSR引物，共扩增出78条清晰易辨的多态性谱带。聚类分析结果表明，利用ISSR技术可将全部供试材料区分开，遗传相似系数变异范围为0．108~0．822，在相似系数为0．51时，12个菌株聚为6个群，其中5个野生菌株各自聚为不同类群，遗传差异较大。

Five wild-type Auricularia strains were identified by ISSR （inter-simple sequence repeat） methodology using seven cultivated Auricularia strains as controls. Eleven ISSR primers, selected from a total of 60 test ISSR primers, amplified 78 distinct polymorphic DNA bands from the 12 Auricularia strains.ISSR similarity coefficients ranged from 0. 108 to 0. 822, and a dendrogram based on UPGMA （unweighted pair group method with arithmetic average） cluster analysis using these values revealed that the 12 strains were clustered into six groups （similarity coefficient 0.51）. The five wild-type strains clustered into five different groups, and the most distinct strain genetically was the wild-type strain, Ausm （similarity coefficient 0. 198）.