摘要
目的探讨亚甲基四氢叶酸还原酶(methylenetetrahydrofolate reductase,简称MTHFR)C677T基因多态性与复发性流产(recurrent abortion,简称RA)发病的相关性。方法采用聚合酶链反应—限制性片段长度多态性(PCR-RFLP)分析RA组60例和对照组60例MTHFRC677T的基因型。结果RA孕妇及胚胎绒毛组织MTHFR677TT基因型频率和T等位基因频率高于对照组(p<0.05);MTHFR677的3种基因型在流产组和对照组的分布差异有统计学意义(p<0.01),流产组CT+TT基因型频率也高于对照组(p<0.05)。结论RA患者和胚胎绒毛组织MTHFR677TT突变与RA有相关性。
Objective To investigate the relationship of the methylenetetrahydrofolate reductase （MTHFR） C677T gene polymorphism to recurrent abortion （RA）. Method MTHFRC677T genotype were analyzed by the technique of Polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） in recurrent abortion group（60 eases） and control group（60 cases）. Results MTHFR677TT genotype frequency and T allele frequencies of RA pregnant women and villi were higher than control group（p〈0.05）. There was a significant difference of the distribution of the 3 genotypes between the recurrent abortion group and the control group. Embryo villi of recurrent abortion MTHFR677CT＋ TT genotype frequencies were higher than control group （p〈0.05）. Conclusion RA patients and embryo villi with the mutation MTHFR677TT each other are related to RA.
出处
《保健医学研究与实践》
2008年第4期4-5,9,共3页
Health Medicine Research and Practice
