他克莫司对骨髓间充质干细胞移植治疗大鼠脊髓损伤的影响

Experimental Research on the Spine Regeneration Using BMSC and FK506 after SCI

目的骨髓间充质干细胞(BMSC)移植同时应用他克莫司(FK506),观察两者对脊髓损伤后大鼠恢复的影响。方法用钳夹法制作大鼠急性脊髓损伤动物模型30只,随机分成3组。A组为单纯损伤组,B组为单纯骨髓干细胞移植组,C组为骨髓干细胞联合FK506组。于伤后1w、2w、4w、6w、8w采用BBB评分。8周后取材,行免疫组化及病理学检查。结果B、C两组大鼠后肢运动功能均有较明显恢复,C组较B组为快,两组统计学上有差异(P<0.05)。A组亦有所恢复,但程度较轻。A组切片,未见神经轴索通过,B、C组可见少量神经轴索样结构。免疫组化显示:A组NF200阳性细胞,GFAP阳性细胞均较B组C组少(P<0.05)。C组Nogo阳性神经元较A、B两组明显减少(P<0.05)。B组NF200阳性细胞,GFAP阳性细胞均较C组少(P<0.05)。结论骨髓间质干细胞移植对于后肢功能的恢复有促进作用,联合应用FK506有协同效果。

Objective To investigate the effect of bone mesenchymal stem cell （BMSC） and tacrolimus （FK506） on spinal cord injury （SCI）. Methods The ring clamp SCI model of rat was constructed, 30 rats were divided randomly into three groups, group A was single SCI group, group B was SCI group treated with BMSC injected, group C was SCI group treated just like group B but with FK.506 which injected through abdominal part. In 1, 2, 4,6,8 weeks post-injury, all animals were evaluated of the hind limb behavior with BBB score test. The immunohistochemistry and pathology, were checked after 8 weeks. Results The outcome of group C surpass the group B. There was significant difference between group B and C （P 〈 0.05 ）. The outcome of group A were similar to group B and C, but the functional recovery degree was smaller than the others. The group A was reported to have no neuroaxonal in the part of lesions but the group B and C was reported to have little. The GFAP-positive cells and NF200-positive cells in the group A were lower than group B and C （P 〈 0.05 ） but Nogo-positive cells in the group C were lower than group A and B（ P 〈0.05）. The GFAP-positive cells and NF200-positive cells in the group B were lower than group C （P 〈0. 05 ）. Conclusions The BMSC possess the promotion effects on spinal cord injured rats, the experimental results showed that with the affiliation of FK506 claim synergic effects on the recovery of injured cord.