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麻风菌抗原诱导IL-12产生的体外研究 被引量：1

Determination of IL-12 secretion induced by antigens of M. leprae in vitro
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摘要 目的:检测麻风菌不同抗原脂蛋白诱导单个核细胞(PBMC)IL-12p40分泌水平。方法:以麻风菌不同抗原刺激体外培养的少菌型(PB)、多菌型(MB)麻风患者及健康接触者PBMC,加入或不加外源性IFN-γ共培养,ELISA检测培养上清的IL-12p40分泌水平。结果:(1)麻风菌胞浆抗原(MLSA)和麻风菌33KDa脂蛋白可诱导健康接触者、PB患者PBMC分泌IL-12p40;(2)外源性IFN-γ可上调健康接触者和PB患者PBMC分泌IL-12p40水平。结论:麻风菌33KDa脂蛋白是PB患者IL-12p40的有效诱导剂,外源性IFN-γ可上调IL-12p40分泌,为麻风病免疫治疗的建立提供了依据。 Objective： To determine the ability of antigens of M. leprae to induce IL - 12p40 secretion on PBMC collected from leprosy patients. Methods： PBMC obtained from leprosy patients and healthy contacts was cultured with various antigens of M. leprae with or without exogenous IFN - 7. The supernatants were collected and IL - 12p40 production was measured by ELISA. Results： （1） MLSA and 33KDa lipopmtein of M. leprae induced IL- 12p40 secretion on PBMC of healthy contacts and PB patients. （2） Exogenous IFN - 7 up - regulated IL - 121940 secretion on PBMC of healthy contacts and PB patients. Conclusion： 33KDa lipoprotein of M. leprae is an effective inducer for secretion of IL- 12p40 and exogenous IFN - 7 can up - regulate IL - 12p40 secretion by PB patients. They are the potential agents which can be used for immuno - therapy in PB leprosy.
作者 陈小华 翁小满
机构地区 首都医科大学附属北京友谊医院北京热带医学研究所
出处 《中国麻风皮肤病杂志》 2008年第11期858-860,共3页 China Journal of Leprosy and Skin Diseases
关键词 麻风病 麻风菌抗原 IL-12P40 leprosy antigens of M. leprae IL- 12p40
分类号 R755 [医药卫生—皮肤病学与性病学]
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参考文献7

  • 1Brightbill HD, Libraty DH, Krutzik SR, et al. Host defense mechanisms triggered by microbial lipoproteins through toll - like receptors. Science 1999;285(5428) :732- 736.
  • 2Maeda Y, Makino M, Crick DC, et al. Novel 33 - kilodalton lipoprotein from mycobacterium leprae. Infect Immun 2002;70( 8): 4106-4111.
  • 3Yamashita Y, Maeda Y, Takeshita F, et al. Role of the polypeptide region of a 33Kda myeobaeterial lipoprotein for efficient IL- 12 production. Cell Immunol 2004 ; 229( 1 ) : 13 - 20.
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同被引文献19

  • 1Montoya D, Modlin RL. [earning from Leprosy Insight into the hu- man innate inunune reslamse. Adv Inmlunol 2010; 105C:I.
  • 2Krutzik SR, Ochoa MT, Sieling PA, et al. Activation and regnJlation of Toll- like receptors 2 and 1 in humml leprosy. Nat Med 2003;9 (5) :525.
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  • 6Berfington WR, Maedonald M, Khadge S, et al. Connnon tx~lymor- phisms in the NOD2 gene region are associated with leprosy and its reactive states. J Infect Dis 2010;201(9):1422.
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  • 10Domelles LN, Pereira- Ferrari L, Messias- Reason I. Mannan- binding lectin plasma levels in leprosy: deficiency confers protec- tion against the lepromatous but not the tuberculOid forms. Clin Exp Immunol 2006; 145(3) :463.

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中国麻风皮肤病杂志
2008年 第11期

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