白藜芦醇对人A375及鼠B16F10黑素瘤细胞增殖及凋亡的影响

Effects of resveratrol on proliferation and apoptosis of A375 and B16F10 melanoma cell lines

目的:探讨白藜芦醇(resveratrol,Res)对人A375及鼠B16F10黑素瘤细胞增殖及凋亡的调节作用。方法:利用体外细胞培养技术、MTT法、流式细胞仪、光学显微镜技术,检测不同浓度的Res对人A375及鼠B16F10细胞增殖及凋亡的影响。结果:Res对人A375及鼠B16F10细胞均有显著的增殖抑制作用,呈剂量效应依赖性关系。结论:Res在体外可抑制恶性黑素瘤(MM)细胞的增殖。

Objective： To determine the effect of resveratrol on cultured human A375 and rat B16F10 melanoma cell lines proliferation and apoptosis in vitro. Methods： The effects of resveratrol on proliferation and apoptosis of human A375 and rat B16F10 melanoma cell lines were assessed using culture technique in vitro, MTT, flow cytometry assay and inverted microscopic observations. Results： Resveratrol obviously suppressed the prolification of A375 and B16F10 melanoma cell lines in a dose - dependent manner. Conclusion： Resveratrol can significantly inhibit the prolification of human A375 and rat B16F10 melanoma cell lines.