抑癌基因RASSF1A微卫星变异与宫颈癌发生发展的关系

Study on the relationship between microsatellite alterations of RASSF1A gene and the development of cervical carcinoma

目的探讨抑癌基因RASSF1A微卫星变异在宫颈癌发生发展中的作用及其与HPV感染的关系。方法选择RASSF1A基因的两个微卫星多态标记位点，采用PCR技术对宫颈组织进行杂合性丢失与微卫星不稳定性的检测，同时检测宫颈组织中HPV16的感染状况。结果两位点的LOH发生率在宫颈癌组织临床分期及病理分级之间差异有统计学意义（P〈0．05）。双位点的LOH及MSI在宫颈癌有无淋巴结转移间差异有统计学意义（P〈0．05）。RASSF1A基因的LOH发生率在HPV16感染阳性组中明显高于阴性组（P〈0．05）。结论RASSF1A基因的改变是宫颈癌发生过程中的较晚期事件，RASSF1A基因的LOH与MSI对于宫颈癌的筛查、早期诊断及判断预后可能具有临床实用价值。RASSF1A基因的LOH与HPV16感染二者共同作用在宫颈癌的发生发展过程中更有意义。

Objective To explore the relationship between microsatellite alterations of RASSF1A gene and the development of cervical carcinoma, and HPV16 infection. Methods Two sites of microsatellite polymorphism of RASSF1A gene were selected, we used polymcrase chain reaction（PCR） technique to detect the LOH and MSI of cervical tissues, and to detect the infection state of HPV16 .Results There were significant differences of LOH rates at the two sites between clinical stage and pathological grade（ P 〈 0.05 ）. Significant differences were noted between the cervical carcinomas with lymph node metastasis and those without lymph node metastasis in regard to their LOH and MSI at the two sites（ P 〈 0.05）. The incidence of LOH of RASSF1A gene was higher in HPV16 （ ＋ ） than that in HPV16 （ - ） （ P 〈 0.05）. Conclusion The change of RASSF1A gone is a relatively late event in cervical carcinomas. The detection of the LOH and MSI of RASSF1A gene might be helpful to the early diagnosis and the screening of cervical carcinoma. It might also be useful for predicting the prognosis of cervical carcinoma. Infection of HPV16 and LOH of RASSF1A gene had reacted together in the development of cervical carcinoma.