8周跑台运动对肥胖大鼠血浆和下丘脑神经肽Y蛋白及其基因表达的影响

The Effect of Chronic Exercise on the Expression of Plasmic and Hypothalamic NPY and NPY mRNA in Obese Rats

目的:探讨运动对肥胖大鼠摄食以及血浆和下丘脑神经肽Y(NPY)的影响。方法:10周营养性肥胖建模成功SD大鼠,分为对照组(C)和运动组(E)。运动组大鼠进行坡度5%、速度20m/min、每天40min的电动跑台训练,共8周。在最后一次运动结束后24小时取材。测试两组大鼠摄食量和血清学指标,包括血糖(GLU)、甘油三酯(TG)、胆固醇(CHOL)、高密度脂蛋白(HDL)和低密度脂蛋白(LDL);血浆和下丘脑NPY蛋白表达以及下丘脑NPY基因表达。结果:E组大鼠摄食量显著低于C组,血脂等血清学指标无显著差异;E组血浆NPY显著低于C组,而下丘脑NPY蛋白和基因表达显著高于C组。结论:8周跑台运动使肥胖大鼠下丘脑NPY合成和分泌增加,这种变化可防止机体能量进一步消耗,有利于恢复。

Objective To investigate the effects of chronic exercise on the food sonsumption, plasmic and hypothalamic NPY concentrations and NPY mRNA in obese rats. Methods Rats with alimentary obesity were randomly divided into two groups. The exercise group ran on the treadmill at a speed of 20m·min^-1 with a slope of 5°, 40min/day for 8 weeks, and food consumption was calculated and GLU, TG, CHOL, HDL, and LDL were measured. Concentrations of NPY were determined by RIA. Expression of NPY in the hypothalamus was detected using RT-PCR. Results After eight-week exercise, food consumption and body weight decreased greatly in exercise group as compared with the control group; Glu and other parameters in serum remained unchanged. The plasma NPY concentrations decreased distinctly, and the concentrations of NPY and NPY mRNA in hypothalamus increased sharply. Conclusions Increased NPY in hypothalamus could activate food consumption, and exercise was able to decrease the concentration of NPY.