摘要
建立了柱前衍生化HPLC法测定朱砂中的汞含量。以二乙基二硫代氨基甲酸钠(NaDDC)为衍生化试剂,样品经消解、衍生化和有机溶剂提取后测定。采用C18色谱柱,流动相为甲醇-水(含0.022mmol/LNaDDC)(90∶10),检测波长为272nm。结果Hg2+在8.4～42.0μg/ml浓度范围内线性关系良好,检测限为4.2ng,平均回收率为99.9%,RSD为2.2%。
An HPLC method with pre-column derivatization was established for the determination of mercury in cinnabar using sodium diethyldithiocarbamate trihydrate （NaDDC） as the derivatizing agent. The samples were treated by HPLC digestion, derivatization and extraction. A Cis column was used with methanol-water （containing 0.022mmol/L NaDDC） （90 ： 10） as the mobile phase at the detection wavelength of 272nm. The calibration curve of Hg^2＋ was linear in the concentration range of 8.4-42.0μg/ml. The detection limit was 4.2ng and the average recovery was 99.9 % with RSD of 2.2%.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2008年第11期849-852,共4页
Chinese Journal of Pharmaceuticals
关键词
汞
朱砂
二乙基二硫代氨基甲酸钠
柱前衍生化
高效液相色谱
测定
mercury
cinnabar
sodium diethyldithiocarbamate trihydrate
pre-column derivatization
HPLC
determination
