山葡萄种内遗传多样性的SSR分析

Analysis of genetic diversity of Vitis amurensis by using SSR markers

利用26对SSR引物对124份山葡萄种质资源的遗传多样性初步分析,等位基因(Na)数平均为3.6923,有效等位基因(Ne)数平均为2.5779,Shannon多样性指数(I)平均为1.0195,基因多样度(Nei)平均为0.5762。野生种质资源的遗传多样性高于选育品种;而野生种质资源中黑龙江省的遗传多样性高,其次是吉林省野生资源,辽宁省野生资源最低。山葡萄种质的遗传相似性系数J为0.224～0.972,平均遗传相似系数为0.588。根据遗传相似性系数进行UPGMA聚类,建立树状聚类图。利用26对SSR标记能将供试的124份山葡萄种质相互区分,在遗传相似性系数0.39处将供试山葡萄种质资源分为6大类群,多数种质的聚类和山葡萄长期生长发育的地理分析研究结果一致。

Twenty-six pairs of SSR molecular markers were used to analyze the genetic diversity of 124 Vitis amurensis germplasms. The preliminary results indicated that the average allelic gene number （Na） was 3.692 3, the effective allelic number （Ne） was 2.5779, and Shannon Index of diversity （Ⅰ） was 1.0195, and the average expected heterozygousity （Nei） was 0.576 2. The genetic diversity of wild germplasms is higher than cuhivars ; the genetic diversity in Heilongjiang is the highest, Jilin is the next, and Liaoning is the lowest in the wild germplasms. The genetic similarity of V. amurensis germplasms coefficient （J） was between 0.224 to 0.972, the average genetic similarity coefficient was 0.588. The clustering dendrogram was constructed by UPGMA method. The 124 V. amurensis germplasms were divided by 26 pairs of SSR molecular markers. The V. amurensis germplasms were divided into 6 major groups while similarity coefficient is 0.39. The majority of germplasms clustering had the same result as the geographical analysis of long-term growth and development of V. amurensis.